Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, <i>Cupriavidus necator</i> IBP/SFU-1, from Various Carbon Sources

• Main Authors: Natalia O. Zhila, Kristina Yu. Sapozhnikova, Evgeniy G. Kiselev, Alexander D. Vasiliev, Ivan V. Nemtsev, Ekaterina I. Shishatskaya, Tatiana G. Volova
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Polymers
• Subjects: <i>Cupriavidus necator</i> IBP/SFU-1; cell growth and PHA synthesis; various carbon sources; PHA composition and properties; polymer films
• Online Access: https://www.mdpi.com/2073-4360/13/18/3142

The bacterial strain isolated from soil was identified as <i>Cupriavidus necator</i> IBP/SFU-1 and investigated as a PHA producer. The strain was found to be able to grow and synthesize PHAs under autotrophic conditions and showed a broad organotrophic potential towards different carbon sources: sugars, glycerol, fatty acids, and plant oils. The highest cell concentrations (7–8 g/L) and PHA contents were produced from oleic acid (78%), fructose, glucose, and palm oil (over 80%). The type of the carbon source influenced the PHA chemical composition and properties: when grown on oleic acid, the strain synthesized the P(3HB-co-3HV) copolymer; on plant oils, the P(3HB-co-3HV-co-3HHx) terpolymer, and on the other substrates, the P(3HB) homopolymer. The type of the carbon source influenced molecular-weight properties of PHAs: P(3HB) synthesized under autotrophic growth conditions, from CO₂, had the highest number-average (290 ± 15 kDa) and weight-average (850 ± 25 kDa) molecular weights and the lowest polydispersity (2.9 ± 0.2); polymers synthesized from organic carbon sources showed increased polydispersity and reduced molecular weight. The carbon source was not found to affect the degree of crystallinity and thermal properties of the PHAs. The type of the carbon source determined not only PHA composition and molecular weight but also surface microstructure and porosity of the polymer films. The new strain can be recommended as a promising P(3HB) producer from palm oil, oleic acid, and sugars (fructose and glucose) and as a producer of P(3HB-co-3HV) from oleic acid and P(3HB-co-3HV-co-3HHx) from palm oil.