Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses
miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry (Vaccinium ashei) tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets fo...
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MDPI AG
2018-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/19/4/983 |
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doaj-db36f5c82e5b4e00b3b9a9b33d36f2f62020-11-24T21:52:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119498310.3390/ijms19040983ijms19040983Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome AnalysesGuangping Li0Yun Wang1Xiaoming Lou2Hailing Li3Changqing Zhang4College of Forest, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;<email>wangyun@jit.edu.cn</email>(Y.W.)College of Horticultural Technology, Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, ChinaCollege of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;<email>wangyun@jit.edu.cn</email>(Y.W.)College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;<email>wangyun@jit.edu.cn</email>(Y.W.)miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry (Vaccinium ashei) tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets for 42 known and 19 novel miRNAs were experimentally validated by degradome sequencing. A functional analysis of these miRNA targets revealed they were associated with diverse biological activities and several pathways, e.g., anthocyanin biosynthesis and cytokinin signal transduction. The data presented herein expand our understanding of the regulation of blueberry miRNAs during floral and fruit development stages. They may also provide new insights into the roles of miRNAs during anthocyanin biosynthesis in blueberry fruits.http://www.mdpi.com/1422-0067/19/4/983blueberrymiRNAsRNA-Seqgene expressiondegradome analysis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Guangping Li Yun Wang Xiaoming Lou Hailing Li Changqing Zhang |
| spellingShingle |
Guangping Li Yun Wang Xiaoming Lou Hailing Li Changqing Zhang Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses International Journal of Molecular Sciences blueberry miRNAs RNA-Seq gene expression degradome analysis |
| author_facet |
Guangping Li Yun Wang Xiaoming Lou Hailing Li Changqing Zhang |
| author_sort |
Guangping Li |
| title |
Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses |
| title_short |
Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses |
| title_full |
Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses |
| title_fullStr |
Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses |
| title_full_unstemmed |
Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses |
| title_sort |
identification of blueberry mirnas and their targets based on high-throughput sequencing and degradome analyses |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2018-03-01 |
| description |
miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry (Vaccinium ashei) tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets for 42 known and 19 novel miRNAs were experimentally validated by degradome sequencing. A functional analysis of these miRNA targets revealed they were associated with diverse biological activities and several pathways, e.g., anthocyanin biosynthesis and cytokinin signal transduction. The data presented herein expand our understanding of the regulation of blueberry miRNAs during floral and fruit development stages. They may also provide new insights into the roles of miRNAs during anthocyanin biosynthesis in blueberry fruits. |
| topic |
blueberry miRNAs RNA-Seq gene expression degradome analysis |
| url |
http://www.mdpi.com/1422-0067/19/4/983 |
| work_keys_str_mv |
AT guangpingli identificationofblueberrymirnasandtheirtargetsbasedonhighthroughputsequencinganddegradomeanalyses AT yunwang identificationofblueberrymirnasandtheirtargetsbasedonhighthroughputsequencinganddegradomeanalyses AT xiaominglou identificationofblueberrymirnasandtheirtargetsbasedonhighthroughputsequencinganddegradomeanalyses AT hailingli identificationofblueberrymirnasandtheirtargetsbasedonhighthroughputsequencinganddegradomeanalyses AT changqingzhang identificationofblueberrymirnasandtheirtargetsbasedonhighthroughputsequencinganddegradomeanalyses |
| _version_ |
1725877500822683648 |