Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains

Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains

Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). T...

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Main Authors: Boglárka Sellyei, Krisztián Bányai, Dániel Bartha, István Hajtós, László Fodor, László Makrai
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2017/1762162
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spelling doaj-db1d9cc793e94e339cd852a79a545dd12020-11-24T23:23:22ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/17621621762162Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis StrainsBoglárka Sellyei0Krisztián Bányai1Dániel Bartha2István Hajtós3László Fodor4László Makrai5CAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, HungaryCAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, HungaryCAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, HungaryGovernment Office for Borsod-Abaúj-Zemplén County, Vologda U. 1, Miskolc 3525, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest 1581, HungaryDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest 1581, HungaryThirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C. pseudotuberculosis were highly similar; the heterogeneity values were low. Genes dnaK, infB, groEL1, and leuA showed marked genetic variation within C. pseudotuberculosis, and strains of the two biotypes of C. pseudotuberculosis could be differentiated. Analysis of the individual genes showed a fairly conservative nature of C. pseudotuberculosis biotype ovis strains. The greatest genetic differentiation was seen in the dnaK and infB genes and concatenations of these two genes were very useful in the genetic separation of the studied strains.http://dx.doi.org/10.1155/2017/1762162
collection DOAJ
language English
format Article
sources DOAJ
author Boglárka Sellyei
Krisztián Bányai
Dániel Bartha
István Hajtós
László Fodor
László Makrai
spellingShingle Boglárka Sellyei
Krisztián Bányai
Dániel Bartha
István Hajtós
László Fodor
László Makrai
Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
BioMed Research International
author_facet Boglárka Sellyei
Krisztián Bányai
Dániel Bartha
István Hajtós
László Fodor
László Makrai
author_sort Boglárka Sellyei
title Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
title_short Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
title_full Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
title_fullStr Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
title_full_unstemmed Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains
title_sort multilocus sequencing of corynebacterium pseudotuberculosis biotype ovis strains
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2017-01-01
description Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C. pseudotuberculosis were highly similar; the heterogeneity values were low. Genes dnaK, infB, groEL1, and leuA showed marked genetic variation within C. pseudotuberculosis, and strains of the two biotypes of C. pseudotuberculosis could be differentiated. Analysis of the individual genes showed a fairly conservative nature of C. pseudotuberculosis biotype ovis strains. The greatest genetic differentiation was seen in the dnaK and infB genes and concatenations of these two genes were very useful in the genetic separation of the studied strains.
url http://dx.doi.org/10.1155/2017/1762162
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