Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat

Background: Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated...

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Main Authors: Zohreh Azizolahi, Hojatollah Alaei, Mahmoud Hosseini, Ali Asghar Pilehvarian
Format: Article
Language:fas
Published: Vesnu Publications 2009-10-01
Series:مجله دانشکده پزشکی اصفهان
Online Access:http://jims.mui.ac.ir/index.php/jims/article/view/286
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spelling doaj-db13e751bdb04e4ebb317400f88be10b2020-11-24T21:55:11ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2009-10-012798450459278Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in RatZohreh Azizolahi0Hojatollah Alaei1Mahmoud Hosseini2Ali Asghar Pilehvarian3MSc, Isfahan Payam-e-Noor University, IsfahanProfessor of Physiology, Department of Physiology, School of Medicine, Isfahan University of Medical Siences, IsfahanAssistant Professor of Physiology, Department of Physiology, School of Medicine, Mashhad University of Medical Siences, MashhadAssistant Professor of Biology, Isfahan Payam-e-Noor University, IsfahanBackground: Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated. Methods: Male wistar rats were anesthetized and stainless style cannula implanted in the locus coeruleus and allowed to recover from surgery. Morphine was injected 3 times in each day for 4 days to induce morphine dependence. The animals divided into 3 groups; control group received saline and case groups received Angiotensin II (1 nanomole) or captopril (30 µg) before naloxone injection. After naloxone injection, morphine withdrawal signs were assessed and compared between groups. Findings: In Angiotensin group, some of withdrawal signs such as wet dog shakes was significantly higher than saline group (P < 0.05). Expression of some of withdrawal signs such as jumping in Angiotensin group was decreased more than saline group (P < 0.01). Also, in captopril group some of withdrawal signs such as wet dog shakes was significantly higher than those of saline group (P < 0.05. Expression of some of withdrawal signs such as writhing posture, teeth chattering, grooming, genital grooming and head shakes were decreased in captopril group more than saline group (P < 0.05). Conclusion: We suggest that Angiotensin II have some roles in opioid withdrawal through locus coeruleus nucleous which contains considerable receptors for it. Key words: Angiotensin II, Morphine, Captopril, Withdrawal, Locus coeruleus, Rat.http://jims.mui.ac.ir/index.php/jims/article/view/286
collection DOAJ
language fas
format Article
sources DOAJ
author Zohreh Azizolahi
Hojatollah Alaei
Mahmoud Hosseini
Ali Asghar Pilehvarian
spellingShingle Zohreh Azizolahi
Hojatollah Alaei
Mahmoud Hosseini
Ali Asghar Pilehvarian
Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
مجله دانشکده پزشکی اصفهان
author_facet Zohreh Azizolahi
Hojatollah Alaei
Mahmoud Hosseini
Ali Asghar Pilehvarian
author_sort Zohreh Azizolahi
title Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
title_short Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
title_full Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
title_fullStr Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
title_full_unstemmed Comparing the Effect of Microinjection of Angiotensin II and Captopril to the Locus Coeruleus on Morphine Withdrawal Signs in Rat
title_sort comparing the effect of microinjection of angiotensin ii and captopril to the locus coeruleus on morphine withdrawal signs in rat
publisher Vesnu Publications
series مجله دانشکده پزشکی اصفهان
issn 1027-7595
1735-854X
publishDate 2009-10-01
description Background: Locus coeruleus is one of important involved sites in morphine withdrawal signs, contains high densities of Angiotensin II receptor binding sites. In this study the effect of microinjection of Angiotensin II and captopril to the Locus coeruleus on morphine withdrawal signs was evaluated. Methods: Male wistar rats were anesthetized and stainless style cannula implanted in the locus coeruleus and allowed to recover from surgery. Morphine was injected 3 times in each day for 4 days to induce morphine dependence. The animals divided into 3 groups; control group received saline and case groups received Angiotensin II (1 nanomole) or captopril (30 µg) before naloxone injection. After naloxone injection, morphine withdrawal signs were assessed and compared between groups. Findings: In Angiotensin group, some of withdrawal signs such as wet dog shakes was significantly higher than saline group (P < 0.05). Expression of some of withdrawal signs such as jumping in Angiotensin group was decreased more than saline group (P < 0.01). Also, in captopril group some of withdrawal signs such as wet dog shakes was significantly higher than those of saline group (P < 0.05. Expression of some of withdrawal signs such as writhing posture, teeth chattering, grooming, genital grooming and head shakes were decreased in captopril group more than saline group (P < 0.05). Conclusion: We suggest that Angiotensin II have some roles in opioid withdrawal through locus coeruleus nucleous which contains considerable receptors for it. Key words: Angiotensin II, Morphine, Captopril, Withdrawal, Locus coeruleus, Rat.
url http://jims.mui.ac.ir/index.php/jims/article/view/286
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