The knee joint loose body as a source of viable autologous human chondrocytes
<p>Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestio...
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PAGEPress Publications
2016-06-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2645 |
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doaj-db134f0fda0b486e8e3542d56c29e0512020-11-25T03:53:23ZengPAGEPress PublicationsEuropean Journal of Histochemistry 1121-760X2038-83062016-06-0160210.4081/ejh.2016.26451509The knee joint loose body as a source of viable autologous human chondrocytesJ. Melrose0Royal North Shore Hospital University of Sydney University of NSW<p>Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.</p>http://www.ejh.it/index.php/ejh/article/view/2645Loose bodyhuman chondrocytestotal knee arthroplasty, repair medicine. |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
J. Melrose |
| spellingShingle |
J. Melrose The knee joint loose body as a source of viable autologous human chondrocytes European Journal of Histochemistry Loose body human chondrocytes total knee arthroplasty, repair medicine. |
| author_facet |
J. Melrose |
| author_sort |
J. Melrose |
| title |
The knee joint loose body as a source of viable autologous human chondrocytes |
| title_short |
The knee joint loose body as a source of viable autologous human chondrocytes |
| title_full |
The knee joint loose body as a source of viable autologous human chondrocytes |
| title_fullStr |
The knee joint loose body as a source of viable autologous human chondrocytes |
| title_full_unstemmed |
The knee joint loose body as a source of viable autologous human chondrocytes |
| title_sort |
knee joint loose body as a source of viable autologous human chondrocytes |
| publisher |
PAGEPress Publications |
| series |
European Journal of Histochemistry |
| issn |
1121-760X 2038-8306 |
| publishDate |
2016-06-01 |
| description |
<p>Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations were undertaken for chondrogenic markers such as aggrecan, and type II collagen. Isolated loose body cells had high viability (≥90% viable), expressed chondrogenic markers (aggrecan, type II collagen) but no type I collagen. Loose bodies may be a useful source of autologous chondrocytes of high viability.</p> |
| topic |
Loose body human chondrocytes total knee arthroplasty, repair medicine. |
| url |
http://www.ejh.it/index.php/ejh/article/view/2645 |
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AT jmelrose thekneejointloosebodyasasourceofviableautologoushumanchondrocytes AT jmelrose kneejointloosebodyasasourceofviableautologoushumanchondrocytes |
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