Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
Background/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
|
Series: | Cellular Physiology and Biochemistry |
Subjects: | |
Online Access: | https://www.karger.com/Article/FullText/485281 |
id |
doaj-dae711b902db4b1487e20501be84a2b8 |
---|---|
record_format |
Article |
spelling |
doaj-dae711b902db4b1487e20501be84a2b82020-11-24T23:55:25ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-0144268270010.1159/000485281485281Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive TractLu ZhangLan-shan HuangGang ChenZhen-bo FengBackground/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive tract is necessary. Methods: Expression profiling data and related-prognostic data of miR-224-5p were acquired from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress, and published literature. The potential target mRNAs of miR-224-5p were predicted using bioinformatics methods and finally annotated using Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results: MiR-224-5p is up-regulated in digestive system cancers (SMD=0.69, 95% CI: 0.43-0.96, P<0.0001) and exhibits a moderate diagnostic ability (AUC=0.84, 95% CI: 0.80-0.87). Our data also demonstrated that miR-224-5p is statistically significantly correlated with overall survival univariate analysis (HR=1.69, 95% CI: 1.15-2.49, P=0.007) and multivariate analysis (HR=2.39, 95% CI: 1.74-3.30, P<0.0001). In total, 388 potential miR-224-5p target mRNAs were predicted by bioinformatics methods. GO annotation analysis revealed that the top terms of miR-224-5p in biological process, cellular component and molecular function were system development, neuron part, and transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding, respectively. Moreover, eight pathways were identified in KEGG pathway enrichment analysis. Conclusions: MiR-224-5p is up-regulated and has the potential to become a diagnostic and prognostic biomarker in digestive system cancers. MiR-224-5p might play vital roles in cancers of the digestive tract but the exact molecular mechanisms need further study and verification.https://www.karger.com/Article/FullText/485281MicrornaMiR-224-5pDigestive system cancersBioinformatics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lu Zhang Lan-shan Huang Gang Chen Zhen-bo Feng |
spellingShingle |
Lu Zhang Lan-shan Huang Gang Chen Zhen-bo Feng Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract Cellular Physiology and Biochemistry Microrna MiR-224-5p Digestive system cancers Bioinformatics |
author_facet |
Lu Zhang Lan-shan Huang Gang Chen Zhen-bo Feng |
author_sort |
Lu Zhang |
title |
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract |
title_short |
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract |
title_full |
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract |
title_fullStr |
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract |
title_full_unstemmed |
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract |
title_sort |
potential targets and clinical value of mir-224-5p in cancers of the digestive tract |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2017-11-01 |
description |
Background/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive tract is necessary. Methods: Expression profiling data and related-prognostic data of miR-224-5p were acquired from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress, and published literature. The potential target mRNAs of miR-224-5p were predicted using bioinformatics methods and finally annotated using Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results: MiR-224-5p is up-regulated in digestive system cancers (SMD=0.69, 95% CI: 0.43-0.96, P<0.0001) and exhibits a moderate diagnostic ability (AUC=0.84, 95% CI: 0.80-0.87). Our data also demonstrated that miR-224-5p is statistically significantly correlated with overall survival univariate analysis (HR=1.69, 95% CI: 1.15-2.49, P=0.007) and multivariate analysis (HR=2.39, 95% CI: 1.74-3.30, P<0.0001). In total, 388 potential miR-224-5p target mRNAs were predicted by bioinformatics methods. GO annotation analysis revealed that the top terms of miR-224-5p in biological process, cellular component and molecular function were system development, neuron part, and transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding, respectively. Moreover, eight pathways were identified in KEGG pathway enrichment analysis. Conclusions: MiR-224-5p is up-regulated and has the potential to become a diagnostic and prognostic biomarker in digestive system cancers. MiR-224-5p might play vital roles in cancers of the digestive tract but the exact molecular mechanisms need further study and verification. |
topic |
Microrna MiR-224-5p Digestive system cancers Bioinformatics |
url |
https://www.karger.com/Article/FullText/485281 |
work_keys_str_mv |
AT luzhang potentialtargetsandclinicalvalueofmir2245pincancersofthedigestivetract AT lanshanhuang potentialtargetsandclinicalvalueofmir2245pincancersofthedigestivetract AT gangchen potentialtargetsandclinicalvalueofmir2245pincancersofthedigestivetract AT zhenbofeng potentialtargetsandclinicalvalueofmir2245pincancersofthedigestivetract |
_version_ |
1725462502585663488 |