Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract

Background/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive...

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Main Authors: Lu Zhang, Lan-shan Huang, Gang Chen, Zhen-bo Feng
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2017-11-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:https://www.karger.com/Article/FullText/485281
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spelling doaj-dae711b902db4b1487e20501be84a2b82020-11-24T23:55:25ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-0144268270010.1159/000485281485281Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive TractLu ZhangLan-shan HuangGang ChenZhen-bo FengBackground/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive tract is necessary. Methods: Expression profiling data and related-prognostic data of miR-224-5p were acquired from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress, and published literature. The potential target mRNAs of miR-224-5p were predicted using bioinformatics methods and finally annotated using Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results: MiR-224-5p is up-regulated in digestive system cancers (SMD=0.69, 95% CI: 0.43-0.96, P<0.0001) and exhibits a moderate diagnostic ability (AUC=0.84, 95% CI: 0.80-0.87). Our data also demonstrated that miR-224-5p is statistically significantly correlated with overall survival univariate analysis (HR=1.69, 95% CI: 1.15-2.49, P=0.007) and multivariate analysis (HR=2.39, 95% CI: 1.74-3.30, P<0.0001). In total, 388 potential miR-224-5p target mRNAs were predicted by bioinformatics methods. GO annotation analysis revealed that the top terms of miR-224-5p in biological process, cellular component and molecular function were system development, neuron part, and transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding, respectively. Moreover, eight pathways were identified in KEGG pathway enrichment analysis. Conclusions: MiR-224-5p is up-regulated and has the potential to become a diagnostic and prognostic biomarker in digestive system cancers. MiR-224-5p might play vital roles in cancers of the digestive tract but the exact molecular mechanisms need further study and verification.https://www.karger.com/Article/FullText/485281MicrornaMiR-224-5pDigestive system cancersBioinformatics
collection DOAJ
language English
format Article
sources DOAJ
author Lu Zhang
Lan-shan Huang
Gang Chen
Zhen-bo Feng
spellingShingle Lu Zhang
Lan-shan Huang
Gang Chen
Zhen-bo Feng
Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
Cellular Physiology and Biochemistry
Microrna
MiR-224-5p
Digestive system cancers
Bioinformatics
author_facet Lu Zhang
Lan-shan Huang
Gang Chen
Zhen-bo Feng
author_sort Lu Zhang
title Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
title_short Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
title_full Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
title_fullStr Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
title_full_unstemmed Potential Targets and Clinical Value of MiR-224-5p in Cancers of the Digestive Tract
title_sort potential targets and clinical value of mir-224-5p in cancers of the digestive tract
publisher Cell Physiol Biochem Press GmbH & Co KG
series Cellular Physiology and Biochemistry
issn 1015-8987
1421-9778
publishDate 2017-11-01
description Background/Aims: MicroRNAs participate in various biological processes in malignant tumors. However, the mechanisms of miR-224-5p in digestive system cancers are not fully understood. A comprehensive investigation of the clinical value and potential targets of miR-224-5p in cancers of the digestive tract is necessary. Methods: Expression profiling data and related-prognostic data of miR-224-5p were acquired from Gene Expression Omnibus, The Cancer Genome Atlas, ArrayExpress, and published literature. The potential target mRNAs of miR-224-5p were predicted using bioinformatics methods and finally annotated using Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Results: MiR-224-5p is up-regulated in digestive system cancers (SMD=0.69, 95% CI: 0.43-0.96, P<0.0001) and exhibits a moderate diagnostic ability (AUC=0.84, 95% CI: 0.80-0.87). Our data also demonstrated that miR-224-5p is statistically significantly correlated with overall survival univariate analysis (HR=1.69, 95% CI: 1.15-2.49, P=0.007) and multivariate analysis (HR=2.39, 95% CI: 1.74-3.30, P<0.0001). In total, 388 potential miR-224-5p target mRNAs were predicted by bioinformatics methods. GO annotation analysis revealed that the top terms of miR-224-5p in biological process, cellular component and molecular function were system development, neuron part, and transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding, respectively. Moreover, eight pathways were identified in KEGG pathway enrichment analysis. Conclusions: MiR-224-5p is up-regulated and has the potential to become a diagnostic and prognostic biomarker in digestive system cancers. MiR-224-5p might play vital roles in cancers of the digestive tract but the exact molecular mechanisms need further study and verification.
topic Microrna
MiR-224-5p
Digestive system cancers
Bioinformatics
url https://www.karger.com/Article/FullText/485281
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