Quantization of total fumonisins produced by Fusarium verticillioides strains in some maize and sorghum genotypes by ELISA

Fusarium verticillioides is one of the most prevalent Fusarium species on maize and sorghum, causing Fusarium ear rot and sorghum grain mold in warm and humid regions of Iran. The pathogen produces potent mycotoxins known as fumonisins. In order to determine mycotoxin (fumonisins) production on...

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Bibliographic Details
Main Authors: Zamani Majid, Feizbaš Tagi Mohamed, Parčamijan Marjam, Rahdžu Vahid
Format: Article
Language:English
Published: Matica srpska 2011-01-01
Series:Zbornik Matice Srpske za Prirodne Nauke
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Online Access:http://www.doiserbia.nb.rs/img/doi/0352-4906/2011/0352-49061121061R.pdf
Description
Summary:Fusarium verticillioides is one of the most prevalent Fusarium species on maize and sorghum, causing Fusarium ear rot and sorghum grain mold in warm and humid regions of Iran. The pathogen produces potent mycotoxins known as fumonisins. In order to determine mycotoxin (fumonisins) production on different maize and sorghum genotypes, a field trial was carried out based on a randomized complete block design with 10 treatments and three replications for each crop at Gorgan station in 2010. The ears of corn plants were inoculated by spore suspension of the mixture of some virulent F. verticillioides isolates using an ear inoculation method (Nail Punch). The sorghum panicles were also inoculated by spraying of spore suspension isolates at f lowering stage. All infected kernels were evaluated by ELISA kits (AgraQuant Fumonisin Kit; Romer Labs, Austria) for their total fumonisins production at the physiological maturing stage. All genotypes showed statistically significant difference in their fumonisin production in Gorgan. The results of fumonisins analysis obtained from ELISA test showed that lines 1 (Resistant) and 3 (Susceptible) with 2.4 ppm and 13.7 ppm had the least and highest amount of total fumonisins respectively among all maize genotypes. Also among all sorghum genotypes, genotypes 10 and 6 with 0.2 ppm and 4.8 ppm had the least and the highest amount of total fumonisins respectively. The results of this experiment demonstrated that fumonisin production level in maize kernels (maybe as maize kernels are the main host of this fungus) was significantly higher than sorghum kernels in Gorgan region.
ISSN:0352-4906