In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA.

In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA.

Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host proteins also bind with great affinity...

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Bibliographic Details
Main Authors:	Ricardo A Orbegozo-Medina, Victoria Martínez-Sernández, María J Perteguer, Ana Hernández-González, Mercedes Mezo, Marta González-Warleta, Fernanda Romarís, Esperanza Paniagua, Teresa Gárate, Florencio M Ubeira
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2019-01-01
Series:	PLoS ONE
Online Access:	https://doi.org/10.1371/journal.pone.0211035

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