Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways
Osteoclasts (OCs) play an important role in osteoporosis, a disease that is mainly characterized by bone loss. In our research, we aimed to identify novel approach for regulating osteoclastogenesis and thereby treating osteoporosis. Previous studies have set a precedent for screening traditional Chi...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.736863/full |
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doaj-dabd9794504548f9ab0160756ab0bec12021-09-24T07:17:38ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-09-011210.3389/fendo.2021.736863736863Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling PathwaysMengbo Zhu0Mengbo Zhu1Wenbin Xu2Jiuzhou Jiang3Yining Wang4Yanjing Guo5Yanjing Guo6Ruijia Yang7Ruijia Yang8Yaqiong Chang9Bin Zhao10Zhenyu Wang11Jianfeng Zhang12Te Wang13Liqin Shangguan14Shaowei Wang15Shaowei Wang16Department of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Biochemistry, Basic Medical College, Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Biochemistry, Basic Medical College, Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaDepartment of Orthopaedics, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaDepartment of Orthopedic, Second Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Biochemistry, Basic Medical College, Shanxi Medical University, Taiyuan, ChinaOsteoclasts (OCs) play an important role in osteoporosis, a disease that is mainly characterized by bone loss. In our research, we aimed to identify novel approach for regulating osteoclastogenesis and thereby treating osteoporosis. Previous studies have set a precedent for screening traditional Chinese herbal extracts for effective inhibitors. Peiminine is an alkaloid extracted from the bulb of Fritillaria thunbergii Miq that reportedly has anticancer and anti-inflammatory effects. Thus, the potential inhibitory effect of peiminine on OC differentiation was investigated via a series of experiments. According to the results, peiminine downregulated the levels of specific genes and proteins in vitro and consequently suppressed OC differentiation and function. Based on these findings, we further investigated the underlying molecular mechanisms and identified the NF-κB and ERK1/2 signaling pathways as potential targets of peiminine. In vivo, peiminine alleviated bone loss in an ovariectomized mouse model.https://www.frontiersin.org/articles/10.3389/fendo.2021.736863/fullpeiminineosteoclastNFATc1NF-κBosteoporosis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mengbo Zhu Mengbo Zhu Wenbin Xu Jiuzhou Jiang Yining Wang Yanjing Guo Yanjing Guo Ruijia Yang Ruijia Yang Yaqiong Chang Bin Zhao Zhenyu Wang Jianfeng Zhang Te Wang Liqin Shangguan Shaowei Wang Shaowei Wang |
| spellingShingle |
Mengbo Zhu Mengbo Zhu Wenbin Xu Jiuzhou Jiang Yining Wang Yanjing Guo Yanjing Guo Ruijia Yang Ruijia Yang Yaqiong Chang Bin Zhao Zhenyu Wang Jianfeng Zhang Te Wang Liqin Shangguan Shaowei Wang Shaowei Wang Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways Frontiers in Endocrinology peiminine osteoclast NFATc1 NF-κB osteoporosis |
| author_facet |
Mengbo Zhu Mengbo Zhu Wenbin Xu Jiuzhou Jiang Yining Wang Yanjing Guo Yanjing Guo Ruijia Yang Ruijia Yang Yaqiong Chang Bin Zhao Zhenyu Wang Jianfeng Zhang Te Wang Liqin Shangguan Shaowei Wang Shaowei Wang |
| author_sort |
Mengbo Zhu |
| title |
Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways |
| title_short |
Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways |
| title_full |
Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways |
| title_fullStr |
Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways |
| title_full_unstemmed |
Peiminine Suppresses RANKL-Induced Osteoclastogenesis by Inhibiting the NFATc1, ERK, and NF-κB Signaling Pathways |
| title_sort |
peiminine suppresses rankl-induced osteoclastogenesis by inhibiting the nfatc1, erk, and nf-κb signaling pathways |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Endocrinology |
| issn |
1664-2392 |
| publishDate |
2021-09-01 |
| description |
Osteoclasts (OCs) play an important role in osteoporosis, a disease that is mainly characterized by bone loss. In our research, we aimed to identify novel approach for regulating osteoclastogenesis and thereby treating osteoporosis. Previous studies have set a precedent for screening traditional Chinese herbal extracts for effective inhibitors. Peiminine is an alkaloid extracted from the bulb of Fritillaria thunbergii Miq that reportedly has anticancer and anti-inflammatory effects. Thus, the potential inhibitory effect of peiminine on OC differentiation was investigated via a series of experiments. According to the results, peiminine downregulated the levels of specific genes and proteins in vitro and consequently suppressed OC differentiation and function. Based on these findings, we further investigated the underlying molecular mechanisms and identified the NF-κB and ERK1/2 signaling pathways as potential targets of peiminine. In vivo, peiminine alleviated bone loss in an ovariectomized mouse model. |
| topic |
peiminine osteoclast NFATc1 NF-κB osteoporosis |
| url |
https://www.frontiersin.org/articles/10.3389/fendo.2021.736863/full |
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