Variation in bovine leptin gene affects milk fatty acid composition in New Zealand Holstein Friesian  ×  Jersey dairy cows
<p>Leptin is a protein hormone secreted from white adipose tissue. It regulates food/feed intake, body weight, immune function and reproduction. In our investigation, the polymerase chain reaction (PCR) amplification coupled with single-strand conformational polymorphism (SSCP) analysis was us...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2021-06-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/64/245/2021/aab-64-245-2021.pdf |
| Summary: | <p>Leptin is a protein hormone secreted from white adipose tissue. It regulates
food/feed intake, body weight, immune function and reproduction. In our
investigation, the polymerase chain reaction (PCR) amplification coupled
with single-strand conformational polymorphism (SSCP) analysis was used to
reveal variation in bovine leptin gene (<i>LEP</i>) in New Zealand (NZ)
Holstein Friesian <span class="inline-formula">×</span> Jersey (HF <span class="inline-formula">×</span> J) dairy cows.
Subsequent sequence analysis of a 430 bp amplicon spanning the entirety of
exon 3 and part of the intron 2 region revealed three variant sequences
(<span class="inline-formula"><i>A</i><sub>3</sub></span>, <span class="inline-formula"><i>B</i><sub>3</sub></span> and <span class="inline-formula"><i>C</i><sub>3</sub></span>) containing a total of five nucleotide substitutions,
all of which have been reported previously. Using general linear
mixed-effect model analyses, the presence of variant <span class="inline-formula"><i>A</i><sub>3</sub></span> (the most common
variant) was associated with a decreased level of C15:1, C18:1 <i>trans</i>-11, C18:1
all <i>trans</i>, C18:2 <i>trans</i>-9, <i>cis</i>-12, C22:0 and C24:0 levels but increased levels of C12:1 and
C13:0 <i>iso</i> (<span class="inline-formula"><i>p</i><i><</i>0.05</span>). Variant <span class="inline-formula"><i>B</i><sub>3</sub></span> was associated with reduced levels
of C6:0, C8:0, C11:0, C13:0 and C20:0 but increased C17:0 <i>iso</i> and C24:0 levels
(<span class="inline-formula"><i>p</i><i><</i>0.05</span>). Variant <span class="inline-formula"><i>C</i><sub>3</sub></span> was associated with decreased C17:0 <i>iso</i>
levels but increased C20:0 (<span class="inline-formula"><i>p</i><i><</i>0.05</span>) levels. In a genotype model, the
<span class="inline-formula"><i>A</i><sub>3</sub><i>B</i><sub>3</sub></span> genotype was associated with increased levels of C22:0 and
C24:0 but decreased C8:0, C10:0, C11:0, C13:0, C15:0 and grouped medium-chain fatty acid (MCFA) levels (<span class="inline-formula"><i>p</i><i><</i>0.05</span>). Genotype
<span class="inline-formula"><i>A</i><sub>3</sub><i>C</i><sub>3</sub></span> was found to be associated with decreased levels of C10:0,
C11:0, C13:0 and grouped MCFA (<span class="inline-formula"><i>p</i><i><</i>0.05</span>). This is the first report of
findings of this kind in NZ HF <span class="inline-formula">×</span> J cows, and they suggest that
variation in exon 3 of bovine leptin gene could be explored as a means of
decreasing the concentration of saturated fatty acids in milk.</p> |
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| ISSN: | 0003-9438 2363-9822 |