Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection

Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV...

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Main Authors: Reza Taherkhani, Fatemeh Farshadpour, Manoochehr Makvandi, Hamid Rajabi Memari, Ali Reza Samarbafzadeh, Nasrin Sharifi, Behrouz Naeimi, Saeed Tajbakhsh, Samad Akbarzadeh
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Journal of Tropical Medicine
Online Access:http://dx.doi.org/10.1155/2015/523560
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spelling doaj-da7bf915b76548158b5c9ebd084dfff62020-11-25T00:59:42ZengHindawi LimitedJournal of Tropical Medicine1687-96861687-96942015-01-01201510.1155/2015/523560523560Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E InfectionReza Taherkhani0Fatemeh Farshadpour1Manoochehr Makvandi2Hamid Rajabi Memari3Ali Reza Samarbafzadeh4Nasrin Sharifi5Behrouz Naeimi6Saeed Tajbakhsh7Samad Akbarzadeh8Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranHealth Research Institute, Infectious and Tropical Disease Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 6135715794, IranDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Ahvaz Shahid Chamran University, Ahvaz 6135715794, IranHealth Research Institute, Infectious and Tropical Disease Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 6135715794, IranDepartment of Nutrition, School of Medicine, Kashan University of Medical Sciences, Kashan 8715988141, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranPersian Gulf Tropical Medicine Research Centre, Bushehr University of Medical Sciences, Bushehr 7514633341, IranBackground. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo.http://dx.doi.org/10.1155/2015/523560
collection DOAJ
language English
format Article
sources DOAJ
author Reza Taherkhani
Fatemeh Farshadpour
Manoochehr Makvandi
Hamid Rajabi Memari
Ali Reza Samarbafzadeh
Nasrin Sharifi
Behrouz Naeimi
Saeed Tajbakhsh
Samad Akbarzadeh
spellingShingle Reza Taherkhani
Fatemeh Farshadpour
Manoochehr Makvandi
Hamid Rajabi Memari
Ali Reza Samarbafzadeh
Nasrin Sharifi
Behrouz Naeimi
Saeed Tajbakhsh
Samad Akbarzadeh
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
Journal of Tropical Medicine
author_facet Reza Taherkhani
Fatemeh Farshadpour
Manoochehr Makvandi
Hamid Rajabi Memari
Ali Reza Samarbafzadeh
Nasrin Sharifi
Behrouz Naeimi
Saeed Tajbakhsh
Samad Akbarzadeh
author_sort Reza Taherkhani
title Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
title_short Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
title_full Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
title_fullStr Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
title_full_unstemmed Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
title_sort cytokine profiles and cell proliferation responses to truncated orf2 protein in iranian patients recovered from hepatitis e infection
publisher Hindawi Limited
series Journal of Tropical Medicine
issn 1687-9686
1687-9694
publishDate 2015-01-01
description Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo.
url http://dx.doi.org/10.1155/2015/523560
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