Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection
Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV...
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doaj-da7bf915b76548158b5c9ebd084dfff62020-11-25T00:59:42ZengHindawi LimitedJournal of Tropical Medicine1687-96861687-96942015-01-01201510.1155/2015/523560523560Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E InfectionReza Taherkhani0Fatemeh Farshadpour1Manoochehr Makvandi2Hamid Rajabi Memari3Ali Reza Samarbafzadeh4Nasrin Sharifi5Behrouz Naeimi6Saeed Tajbakhsh7Samad Akbarzadeh8Department of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranHealth Research Institute, Infectious and Tropical Disease Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 6135715794, IranDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Ahvaz Shahid Chamran University, Ahvaz 6135715794, IranHealth Research Institute, Infectious and Tropical Disease Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 6135715794, IranDepartment of Nutrition, School of Medicine, Kashan University of Medical Sciences, Kashan 8715988141, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranDepartment of Microbiology and Parasitology, School of Medicine, Bushehr University of Medical Sciences, Bushehr 7514633341, IranPersian Gulf Tropical Medicine Research Centre, Bushehr University of Medical Sciences, Bushehr 7514633341, IranBackground. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo.http://dx.doi.org/10.1155/2015/523560 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh |
spellingShingle |
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection Journal of Tropical Medicine |
author_facet |
Reza Taherkhani Fatemeh Farshadpour Manoochehr Makvandi Hamid Rajabi Memari Ali Reza Samarbafzadeh Nasrin Sharifi Behrouz Naeimi Saeed Tajbakhsh Samad Akbarzadeh |
author_sort |
Reza Taherkhani |
title |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_short |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_full |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_fullStr |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_full_unstemmed |
Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection |
title_sort |
cytokine profiles and cell proliferation responses to truncated orf2 protein in iranian patients recovered from hepatitis e infection |
publisher |
Hindawi Limited |
series |
Journal of Tropical Medicine |
issn |
1687-9686 1687-9694 |
publishDate |
2015-01-01 |
description |
Background. The aim of this study was to evaluate hepatitis E virus (HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine. Methods. A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γ ELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups. Results. The truncated ORF2 protein was able to induce IFN-γ ELISPOT and cell proliferation responses and to produce significant amounts of IFN-γ and IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokines in vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines. Conclusion. The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this protein in vivo. |
url |
http://dx.doi.org/10.1155/2015/523560 |
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