Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded

Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded

Systematically understanding the sequence determinants to substrate specificity for enzymes has implications in areas from evolutionary biology to biocatalysis. Here, Whitehead and colleagues generate and analyse near-comprehensive single-mutation fitness landscapes for an amidase with three differe...

Full description

Bibliographic Details
Main Authors: Emily E. Wrenbeck, Laura R. Azouz, Timothy A. Whitehead
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms15695
id doaj-da48e47ebe3f4c798a64a10a429756a4
record_format Article
spelling doaj-da48e47ebe3f4c798a64a10a429756a42021-05-11T07:56:24ZengNature Publishing GroupNature Communications2041-17232017-06-018111010.1038/ncomms15695Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encodedEmily E. Wrenbeck0Laura R. Azouz1Timothy A. Whitehead2Department of Chemical Engineering and Materials Science, Michigan State UniversityDepartment of Chemical Engineering and Materials Science, Michigan State UniversityDepartment of Chemical Engineering and Materials Science, Michigan State UniversitySystematically understanding the sequence determinants to substrate specificity for enzymes has implications in areas from evolutionary biology to biocatalysis. Here, Whitehead and colleagues generate and analyse near-comprehensive single-mutation fitness landscapes for an amidase with three different substrates.https://doi.org/10.1038/ncomms15695
collection DOAJ
language English
format Article
sources DOAJ
author Emily E. Wrenbeck
Laura R. Azouz
Timothy A. Whitehead
spellingShingle Emily E. Wrenbeck
Laura R. Azouz
Timothy A. Whitehead
Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
Nature Communications
author_facet Emily E. Wrenbeck
Laura R. Azouz
Timothy A. Whitehead
author_sort Emily E. Wrenbeck
title Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
title_short Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
title_full Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
title_fullStr Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
title_full_unstemmed Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
title_sort single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-06-01
description Systematically understanding the sequence determinants to substrate specificity for enzymes has implications in areas from evolutionary biology to biocatalysis. Here, Whitehead and colleagues generate and analyse near-comprehensive single-mutation fitness landscapes for an amidase with three different substrates.
url https://doi.org/10.1038/ncomms15695
work_keys_str_mv AT emilyewrenbeck singlemutationfitnesslandscapesforanenzymeonmultiplesubstratesrevealspecificityisgloballyencoded
AT laurarazouz singlemutationfitnesslandscapesforanenzymeonmultiplesubstratesrevealspecificityisgloballyencoded
AT timothyawhitehead singlemutationfitnesslandscapesforanenzymeonmultiplesubstratesrevealspecificityisgloballyencoded
_version_ 1721451335960756224

Similar Items