| Summary: | Knowing the normal cleanroom microbiota is the basis for ensuring microbiological quality; assess changes and the introduction of new sampling methods. During our study, we prepared a catalogue of cleanroom microorganisms located in four different cleanrooms in University Clinical Centre Ljubljana Pharmacy. Catalogue is prepared as a basis for assessing the suitability of the new rapid microbiological method and subsequent correlation of the results of both methods. The results of our study showed that 78% of isolated bacteria are Gram-positive. However, in more than 70% isolated bacteria were the part of the normal human microbiota, 10–15% of the microorganisms originated from the air, mainly spore-forming bacteria of the genus Bacillus and fungi, and 5–10% of the Gram-negative microorganisms that originated from the water and represent the potential endotoxins (pyrogens). Keywords: Cleanroom, Environmental monitoring, Microbiota, Microorganisms, Colony forming unit, Bioluminescence, Adenosine triphosphate
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