A new, simple and rapid HPLC method for determination of chlortetracycline in pig solid manure

• Main Authors: Tian Wang, Ruicheng Wei, Ming Chen, Ran Wang
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2010-04-01
• Series: Italian Journal of Animal Science
• Subjects: Analytical method, Chlortetracycline, High-performance liquid chromatography (HPLC), Pig solid manure
• Online Access: http://www.aspajournal.it/index.php/ijas/article/view/1740

Chlortetracycline (CTC) is the most frequently used antibacterial in today’s swine production for disease treatment and growth promotion. It is not absorbed completely and a considerable amount is excreted into manure when CTC is administrated to animal. For this reason there is high concern on potential risks that manure-derived CTC enters into the surface water and selects for resistant microorganism. In order to support the monitoring of CTC and study its fate in manure and environment, a simple and rapid analytical procedure was developed to determine chlortetracycline (CTC) in pig solid manure. The extraction solution, composed of acetone-4mol.L-1 hydrochloric acid-water (13:1:6, v/v/v), was mixed into the manure sample, and the solution was pH adjusted at 2.0-2.2 before extraction for 20 min. Chromatographic separation was carried out on a C18 column at 375 nm with UV detector, using isocratic elution with oxalic acid-methanol-acetonitrile (72:14:14), v/v/v) as mobile phase at room temperature. Recoveries of CTC spiked samples at the levels of 1.0 mg.kg-1, 2.0 mg.kg-1 and 10.0 mg.kg-1 were 92.2%-100.73% with relative standard deviation (RSD) of 2.81%-7.11% intra-day and 1.34%-3.25% inter-day, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.2 mg.kg-1 and 0.8 mg.kg-1, respectively. The procedure developed in this study was tested to be simple, sensitive and suitable for the determination and screening of CTC in pig solid manure samples.