Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.

Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission...

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Main Authors: Michael J Mlodzianoski, Nikki M Curthoys, Mudalige S Gunewardene, Sean Carter, Samuel T Hess
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0147506
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spelling doaj-d9dbdfe132424ebb9146875ee9fc57482021-03-03T19:56:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e014750610.1371/journal.pone.0147506Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.Michael J MlodzianoskiNikki M CurthoysMudalige S GunewardeneSean CarterSamuel T HessLocalization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.https://doi.org/10.1371/journal.pone.0147506
collection DOAJ
language English
format Article
sources DOAJ
author Michael J Mlodzianoski
Nikki M Curthoys
Mudalige S Gunewardene
Sean Carter
Samuel T Hess
spellingShingle Michael J Mlodzianoski
Nikki M Curthoys
Mudalige S Gunewardene
Sean Carter
Samuel T Hess
Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
PLoS ONE
author_facet Michael J Mlodzianoski
Nikki M Curthoys
Mudalige S Gunewardene
Sean Carter
Samuel T Hess
author_sort Michael J Mlodzianoski
title Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
title_short Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
title_full Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
title_fullStr Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
title_full_unstemmed Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
title_sort super-resolution imaging of molecular emission spectra and single molecule spectral fluctuations.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2016-01-01
description Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.
url https://doi.org/10.1371/journal.pone.0147506
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AT mudaligesgunewardene superresolutionimagingofmolecularemissionspectraandsinglemoleculespectralfluctuations
AT seancarter superresolutionimagingofmolecularemissionspectraandsinglemoleculespectralfluctuations
AT samuelthess superresolutionimagingofmolecularemissionspectraandsinglemoleculespectralfluctuations
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