The use of the Petri net method in the simulation modeling of mitochondrial swelling

• Main Authors: Yu. V. Danylovych, A. Y. Chunikhin, G. V. Danylovych, O. V. Kolomiets
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2016-08-01
• Series: Ukrainian Biochemical Journal
• Subjects: calcium; mitochondria; permeability transition pore
• Online Access: http://ukrbiochemjournal.org/wp-content/uploads/2016/09/Danylovych_4_16.pdf

Using photon correlation spectroscopy, which allows investigating changes in the hydrodynamic dia­meter of the particles in suspension, it was shown that ultrahigh concentrations of Ca2+ (over 10 mM) induce swelling of isolated mitochondria. An increase in hydrodynamic diameter was caused by an increase of non-specific mitochondrial membrane permeability to Ca ions, matrix Ca2+ overload, activation of ATP- and Ca2+-sensitive K+-channels, as well as activation of cyclosporin-sensitive permeability transition pore. To formalize the experimental data and to assess conformity of experimental results with theoretical predictions we developed a simulation model using the hybrid functional Petri net method.