A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects
The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-09-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/03115 |
| id |
doaj-d9d70a01c352411893d859123f516fdc |
|---|---|
| record_format |
Article |
| spelling |
doaj-d9d70a01c352411893d859123f516fdc2021-05-04T23:27:08ZengeLife Sciences Publications LtdeLife2050-084X2014-09-01310.7554/eLife.03115A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defectsAlexandre Pfister0Marie Barberon1Julien Alassimone2Lothar Kalmbach3Yuree Lee4Joop EM Vermeer5Misako Yamazaki6Guowei Li7Christophe Maurel8Junpei Takano9Takehiro Kamiya10David E Salt11Daniele Roppolo12Niko Geldner13Department of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandBiochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, FranceBiochimie et Physiologie Moléculaire des Plantes, CNRS/INRA/SupAgro/Université Montpellier, Montpellier, FranceResearch Faculty of Agriculture, Hokkaido University, Hokkaido, JapanInstitute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United KingdomInstitute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, United KingdomDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandDepartment of Plant Molecular Biology, University of Lausanne, Lausanne, SwitzerlandThe endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)—major players of endodermal differentiation—into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.https://elifesciences.org/articles/03115root biologypolarityplant nutrition |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Alexandre Pfister Marie Barberon Julien Alassimone Lothar Kalmbach Yuree Lee Joop EM Vermeer Misako Yamazaki Guowei Li Christophe Maurel Junpei Takano Takehiro Kamiya David E Salt Daniele Roppolo Niko Geldner |
| spellingShingle |
Alexandre Pfister Marie Barberon Julien Alassimone Lothar Kalmbach Yuree Lee Joop EM Vermeer Misako Yamazaki Guowei Li Christophe Maurel Junpei Takano Takehiro Kamiya David E Salt Daniele Roppolo Niko Geldner A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects eLife root biology polarity plant nutrition |
| author_facet |
Alexandre Pfister Marie Barberon Julien Alassimone Lothar Kalmbach Yuree Lee Joop EM Vermeer Misako Yamazaki Guowei Li Christophe Maurel Junpei Takano Takehiro Kamiya David E Salt Daniele Roppolo Niko Geldner |
| author_sort |
Alexandre Pfister |
| title |
A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| title_short |
A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| title_full |
A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| title_fullStr |
A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| title_full_unstemmed |
A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| title_sort |
receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects |
| publisher |
eLife Sciences Publications Ltd |
| series |
eLife |
| issn |
2050-084X |
| publishDate |
2014-09-01 |
| description |
The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)—major players of endodermal differentiation—into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane. |
| topic |
root biology polarity plant nutrition |
| url |
https://elifesciences.org/articles/03115 |
| work_keys_str_mv |
AT alexandrepfister areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT mariebarberon areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT julienalassimone areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT lotharkalmbach areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT yureelee areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT joopemvermeer areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT misakoyamazaki areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT guoweili areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT christophemaurel areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT junpeitakano areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT takehirokamiya areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT davidesalt areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT danieleroppolo areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT nikogeldner areceptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT alexandrepfister receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT mariebarberon receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT julienalassimone receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT lotharkalmbach receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT yureelee receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT joopemvermeer receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT misakoyamazaki receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT guoweili receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT christophemaurel receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT junpeitakano receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT takehirokamiya receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT davidesalt receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT danieleroppolo receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects AT nikogeldner receptorlikekinasemutantwithabsentendodermaldiffusionbarrierdisplaysselectivenutrienthomeostasisdefects |
| _version_ |
1721477032682979328 |