2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism
Abstract Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then...
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2021-06-01
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doaj-d9d18904b4ea49aab08d5971979dbd202021-06-13T11:28:07ZengNature Publishing GroupCell Discovery2056-59682021-06-017111210.1038/s41421-021-00273-22,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolismWensi Meng0Lijie Zhang1Menghao Cao2Yongjia Zhang3Yipeng Zhang4Ping Li5Zhaoqi Kang6Shiting Guo7Ping Xu8Cuiqing Ma9Chao Gao10State Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityState Key Laboratory of Microbial Technology, Shandong UniversityAbstract Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions.https://doi.org/10.1038/s41421-021-00273-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wensi Meng Lijie Zhang Menghao Cao Yongjia Zhang Yipeng Zhang Ping Li Zhaoqi Kang Shiting Guo Ping Xu Cuiqing Ma Chao Gao |
spellingShingle |
Wensi Meng Lijie Zhang Menghao Cao Yongjia Zhang Yipeng Zhang Ping Li Zhaoqi Kang Shiting Guo Ping Xu Cuiqing Ma Chao Gao 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism Cell Discovery |
author_facet |
Wensi Meng Lijie Zhang Menghao Cao Yongjia Zhang Yipeng Zhang Ping Li Zhaoqi Kang Shiting Guo Ping Xu Cuiqing Ma Chao Gao |
author_sort |
Wensi Meng |
title |
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism |
title_short |
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism |
title_full |
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism |
title_fullStr |
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism |
title_full_unstemmed |
2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism |
title_sort |
2,3-butanediol synthesis from glucose supplies nadh for elimination of toxic acetate produced during overflow metabolism |
publisher |
Nature Publishing Group |
series |
Cell Discovery |
issn |
2056-5968 |
publishDate |
2021-06-01 |
description |
Abstract Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate here that acetate increases and then decreases during the growth of a bacterium Enterobacter cloacae subsp. dissolvens SDM. Both bifunctional acetaldehyde/ethanol dehydrogenase AdhE-catalyzed ethanol production and acetate-induced 2,3-BD biosynthesis are indispensable for the elimination of acetate generated during overflow metabolism. 2,3-BD biosynthesis from glucose supplies NADH required for acetate elimination via AdhE-catalyzed ethanol production. The coupling strategy involving 2,3-BD biosynthesis and ethanol production is widely distributed in bacteria and is important for toxic acetate elimination. Finally, we realized the co-production of ethanol and acetoin from chitin, the second most abundant natural biopolymer whose catabolism involves inevitable acetate production through the coupling acetate elimination strategy. The synthesis of a non-toxic chemical such as 2,3-BD may be viewed as a unique overflow metabolism with desirable metabolic functions. |
url |
https://doi.org/10.1038/s41421-021-00273-2 |
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