Thin-Layer Film with an Incorporated Pyrazoloquinoline Derivative as a Fluorescent Sensor for Nucleotides

• Main Authors: Piotr Cywiński, Barbara Wandelt, Andrzej Danel
• Format: Article
• Language: English
• Published: Hindawi - SAGE Publishing 2004-11-01
• Series: Adsorption Science & Technology
• Online Access: https://doi.org/10.1260/0263617043026505

A thin-layer film comprising poly(methyl methacrylate) with an incorporated pyrazolo[3,4- b ]quinoline derivative was prepared. The dye was anchored onto the polymer film to form a molecular assembly at the surface. Fluorescence quenching of this thin-layer sensory system was observed when the film was incubated in the presence of the nucleotide guanosine-3′,5′-cyclic monophosphate. Steady-state and time-resolved fluorescence techniques were used to study the fluorescence quenching of this sensory system in the presence of nucleotides. The results suggest that quenching of a pyrazoloquinoline fluorosensor by nucleotide anions cannot be described simply by a collision mechanism employing the Stern-Volmer equation, but there is a possibility of the formation of a more stable association. This type of sensing polymer layer plays a role in biotechnology because it can be immobilized at the end of a fibre optic and used for measuring nucleotide concentrations within a biological system.