Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified b...

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Bibliographic Details
Main Authors: Aiping Song, Peiling Li, Jiafu Jiang, Sumei Chen, Huiyun Li, Jun Zeng, Yafeng Shao, Lu Zhu, Zhaohe Zhang, Fadi Chen
Format: Article
Language:English
Published: MDPI AG 2014-08-01
Series:International Journal of Molecular Sciences
Subjects:
Chrysanthemum morifolium
phylogenetic analysis
stress response
transcription pattern
Online Access:http://www.mdpi.com/1422-0067/15/8/14442
Description
Summary:WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior.
ISSN:1422-0067

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