Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obt...

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Main Authors: Jorge Arca-Suárez, Fátima Galán-Sánchez, Pilar Marin-Casanova, Manuel A Rodríguez-Iglesias
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5608331?pdf=render
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spelling doaj-d9887831f89c4e38aa48222cbebe943b2020-11-24T20:45:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018522910.1371/journal.pone.0185229Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.Jorge Arca-SuárezFátima Galán-SánchezPilar Marin-CasanovaManuel A Rodríguez-IglesiasWe developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.http://europepmc.org/articles/PMC5608331?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jorge Arca-Suárez
Fátima Galán-Sánchez
Pilar Marin-Casanova
Manuel A Rodríguez-Iglesias
spellingShingle Jorge Arca-Suárez
Fátima Galán-Sánchez
Pilar Marin-Casanova
Manuel A Rodríguez-Iglesias
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
PLoS ONE
author_facet Jorge Arca-Suárez
Fátima Galán-Sánchez
Pilar Marin-Casanova
Manuel A Rodríguez-Iglesias
author_sort Jorge Arca-Suárez
title Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
title_short Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
title_full Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
title_fullStr Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
title_full_unstemmed Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
title_sort direct identification of microorganisms from thioglycolate broth by maldi-tof ms.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
url http://europepmc.org/articles/PMC5608331?pdf=render
work_keys_str_mv AT jorgearcasuarez directidentificationofmicroorganismsfromthioglycolatebrothbymalditofms
AT fatimagalansanchez directidentificationofmicroorganismsfromthioglycolatebrothbymalditofms
AT pilarmarincasanova directidentificationofmicroorganismsfromthioglycolatebrothbymalditofms
AT manuelarodrigueziglesias directidentificationofmicroorganismsfromthioglycolatebrothbymalditofms
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