Study of vac A genotypes of H.pylori Isolated from Patients with the Upper Gastrointestinal Diseases by PCR
Background Helicobacter pylori (H. pylori) is a gram negative curved bacilli colonized in the human stomach. It causes duodenal ulcer, gastritis and is associated with adenocarinoma. There might be a possible relationship between cagA, vacA and ice a genes and clinical outcomes. The aim of t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Vesnu Publications
2009-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/195 |
| Summary: | Background
Helicobacter pylori (H. pylori) is a gram negative curved bacilli colonized in the human stomach. It causes duodenal ulcer, gastritis and is associated with adenocarinoma. There might be a possible relationship between cagA, vacA and ice a genes and clinical outcomes. The aim of this study was to identify the frequency of vacA genotypes of H. pylori isolated from the upper gastrointestinal.
Methods
One hundred H. pylori strains were isolated from patients with different gastrointestinal disease who admitted to Isfahan Al-zahra hospital. Vac A alleles were typed using PCR with specific primers.
Findings
There were four Vac A mosaicsms, including 28 for s1a/m1 (28%), 23 for s1b/m1 (23%), 26 for s1a/m2 (26%) and 23 for s1b/m2 (23%), s2 form was not found.
Conclusion
The results showed there is no significant relationship between different genotypes of vacA and the related diseases.
Key words: Helicobacter pylori, vac A, Adenocarinoma, Duodenal ulcer, Normal /Gastritis, PCR |
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| ISSN: | 1027-7595 1735-854X |