Development and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever viruses

Development and evaluation of duplex TaqMan real-time PCR assay for detection and differentiation of wide-type and MGF505-2R gene-deleted African swine fever viruses

Abstract Background African swine fever (ASF) is the most important disease to the pigs and cause serious economic losses to the countries with large-scale swine production. Vaccines are recognized as the most useful tool to prevent and control ASF virus (ASFV) infection. Currently, the MGF505 and M...

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Bibliographic Details
Main Authors: Zhenhua Guo, Kunpeng Li, Songlin Qiao, Xin-xin Chen, Ruiguang Deng, Gaiping Zhang
Format: Article
Language:English
Published: BMC 2020-11-01
Series:BMC Veterinary Research
Subjects:
African swine fever virus
Duplex real-time PCR
Differential diagnosis
Gene-deleted strains
Online Access:http://link.springer.com/article/10.1186/s12917-020-02639-2

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