Real Time Monitoring of NADPH Concentrations in Corynebacterium glutamicum and Escherichia coli via the Genetically Encoded Sensor mBFP

Real Time Monitoring of NADPH Concentrations in Corynebacterium glutamicum and Escherichia coli via the Genetically Encoded Sensor mBFP

Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial production strains and process optimization. mBFP was described as metagenomics derived, blue fluorescent protein showing NADPH-dependent fluorescence. Characterization of mBFP showed a high specificity for...

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Bibliographic Details
Main Authors: Oliver Goldbeck, Alexander W. Eck, Gerd M. Seibold
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Microbiology
Subjects:
Corynebacterium glutamicum
Escherichia coli
NADPH
redox state
biosensor
short chain dehydrogenase
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.02564/full

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