Current and Emerging Methods for the Synthesis of Single-Stranded DNA

Current and Emerging Methods for the Synthesis of Single-Stranded DNA

Methods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA. Some approaches allow...

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Main Authors: Min Hao, Jianjun Qiao, Hao Qi
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Genes
Subjects:
ssdna
chemical synthesis
enzyme synthesis
bacterial-based synthesis
Online Access:https://www.mdpi.com/2073-4425/11/2/116
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spelling doaj-d8d18d4b68a7498b8aab71571cbdb2e32020-11-25T01:30:14ZengMDPI AGGenes2073-44252020-01-0111211610.3390/genes11020116genes11020116Current and Emerging Methods for the Synthesis of Single-Stranded DNAMin Hao0Jianjun Qiao1Hao Qi2School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaMethods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA. Some approaches allow the synthesis of freely chosen user-defined ssDNA sequences to overcome the restrictions and limitations of different length, purity, and yield. In this perspective, we provide an overview of the representative ssDNA production strategies and their most significant challenges to enable the readers to make informed choices of synthesis methods and enhance the availability of increasingly inexpensive synthetic ssDNA. We also aim to stimulate a broader interest in the continued development of efficient ssDNA synthesis techniques and improve their applications in future research.https://www.mdpi.com/2073-4425/11/2/116ssdnachemical synthesisenzyme synthesisbacterial-based synthesis
collection DOAJ
language English
format Article
sources DOAJ
author Min Hao
Jianjun Qiao
Hao Qi
spellingShingle Min Hao
Jianjun Qiao
Hao Qi
Current and Emerging Methods for the Synthesis of Single-Stranded DNA
Genes
ssdna
chemical synthesis
enzyme synthesis
bacterial-based synthesis
author_facet Min Hao
Jianjun Qiao
Hao Qi
author_sort Min Hao
title Current and Emerging Methods for the Synthesis of Single-Stranded DNA
title_short Current and Emerging Methods for the Synthesis of Single-Stranded DNA
title_full Current and Emerging Methods for the Synthesis of Single-Stranded DNA
title_fullStr Current and Emerging Methods for the Synthesis of Single-Stranded DNA
title_full_unstemmed Current and Emerging Methods for the Synthesis of Single-Stranded DNA
title_sort current and emerging methods for the synthesis of single-stranded dna
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2020-01-01
description Methods for synthesizing arbitrary single-strand DNA (ssDNA) fragments are rapidly becoming fundamental tools for gene editing, DNA origami, DNA storage, and other applications. To meet the rising application requirements, numerous methods have been developed to produce ssDNA. Some approaches allow the synthesis of freely chosen user-defined ssDNA sequences to overcome the restrictions and limitations of different length, purity, and yield. In this perspective, we provide an overview of the representative ssDNA production strategies and their most significant challenges to enable the readers to make informed choices of synthesis methods and enhance the availability of increasingly inexpensive synthetic ssDNA. We also aim to stimulate a broader interest in the continued development of efficient ssDNA synthesis techniques and improve their applications in future research.
topic ssdna
chemical synthesis
enzyme synthesis
bacterial-based synthesis
url https://www.mdpi.com/2073-4425/11/2/116
work_keys_str_mv AT minhao currentandemergingmethodsforthesynthesisofsinglestrandeddna
AT jianjunqiao currentandemergingmethodsforthesynthesisofsinglestrandeddna
AT haoqi currentandemergingmethodsforthesynthesisofsinglestrandeddna
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