An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells

An Endogenously Tagged Fluorescent Fusion Protein Library in Mouse Embryonic Stem Cells

Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated a library of over 200 endo...

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Bibliographic Details
Main Authors: Arigela Harikumar, Raghu Ram Edupuganti, Matan Sorek, Gajendra Kumar Azad, Styliani Markoulaki, Petra Sehnalová, Soňa Legartová, Eva Bártová, Shlomit Farkash-Amar, Rudolf Jaenisch, Uri Alon, Eran Meshorer
Format: Article
Language:English
Published: Elsevier 2017-10-01
Series:Stem Cell Reports
Subjects:
embryonic stem cells
imaging
live imaging
fluorescence
differentiation
pluripotency
GFP
microscopy
DNA damage
protein dynamics
Online Access:http://www.sciencedirect.com/science/article/pii/S221367111730379X

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http://www.sciencedirect.com/science/article/pii/S221367111730379X

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