Continuous and discrete phasor analysis of binned or time-gated periodic decays

Continuous and discrete phasor analysis of binned or time-gated periodic decays

The time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponentia...

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Main Author: Xavier Michalet
Format: Article
Language:English
Published: AIP Publishing LLC 2021-03-01
Series:AIP Advances
Online Access:http://dx.doi.org/10.1063/5.0027834
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spelling doaj-d8abdede5b834169a4ac359f0e238d402021-04-02T15:45:28ZengAIP Publishing LLCAIP Advances2158-32262021-03-01113035331035331-3710.1063/5.0027834Continuous and discrete phasor analysis of binned or time-gated periodic decaysXavier Michalet0Department of Chemistry and Biochemistry, 607 Charles E. Young Drive E., Los Angeles, California 90095, USAThe time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of instrument response function offset, decay truncation, and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented, and an illustration of the new concepts with examples from the literature concludes this work.http://dx.doi.org/10.1063/5.0027834
collection DOAJ
language English
format Article
sources DOAJ
author Xavier Michalet
spellingShingle Xavier Michalet
Continuous and discrete phasor analysis of binned or time-gated periodic decays
AIP Advances
author_facet Xavier Michalet
author_sort Xavier Michalet
title Continuous and discrete phasor analysis of binned or time-gated periodic decays
title_short Continuous and discrete phasor analysis of binned or time-gated periodic decays
title_full Continuous and discrete phasor analysis of binned or time-gated periodic decays
title_fullStr Continuous and discrete phasor analysis of binned or time-gated periodic decays
title_full_unstemmed Continuous and discrete phasor analysis of binned or time-gated periodic decays
title_sort continuous and discrete phasor analysis of binned or time-gated periodic decays
publisher AIP Publishing LLC
series AIP Advances
issn 2158-3226
publishDate 2021-03-01
description The time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of instrument response function offset, decay truncation, and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented, and an illustration of the new concepts with examples from the literature concludes this work.
url http://dx.doi.org/10.1063/5.0027834
work_keys_str_mv AT xaviermichalet continuousanddiscretephasoranalysisofbinnedortimegatedperiodicdecays
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