Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds
A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment for cells. In this study, porous antibacterial collagenous scaffolds contain...
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MDPI AG
2020-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/10/1971 |
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doaj-d860a9cb4f5f4e73ba87bd76fa0e81c42020-11-25T03:28:56ZengMDPI AGNanomaterials2079-49912020-10-01101971197110.3390/nano10101971Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn WoundsJana Dorazilová0Johana Muchová1Kristýna Šmerková2Silvia Kočiová3Pavel Diviš4Pavel Kopel5Radek Veselý6Veronika Pavliňáková7Vojtěch Adam8Lucy Vojtová9CEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicDepartment of Traumatology at the Medical Faculty, Masaryk University and Trauma Hospital of Brno, Ponavka 6, 662 50 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicCEITEC—Central European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00 Brno, Czech RepublicA highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment for cells. In this study, porous antibacterial collagenous scaffolds containing chitosan and selenium nanoparticles (SeNPs) as antibacterial agents were studied. The addition of antibacterial agents increased the application potential of the material for infected and chronic wounds. The morphology, swelling, biodegradation, and antibacterial activity of collagen-based scaffolds were characterized systematically to investigate the overall impact of the antibacterial additives. The additives visibly influenced the morphology, water‑retaining properties as well as the stability of the materials in the presence of collagenase enzymes. Even at concentrations as low as 5 ppm of SeNPs, modified polymeric scaffolds showed considerable inhibition activity towards Gram-positive bacterial strains such as <i>Staphylococcus aureus</i> and methicillin-resistant <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> in a dose-dependent manner.https://www.mdpi.com/2079-4991/10/10/1971tissue engineeringdrug releasefreeze-dryingcollagenchitosanselenium nanoparticles |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jana Dorazilová Johana Muchová Kristýna Šmerková Silvia Kočiová Pavel Diviš Pavel Kopel Radek Veselý Veronika Pavliňáková Vojtěch Adam Lucy Vojtová |
| spellingShingle |
Jana Dorazilová Johana Muchová Kristýna Šmerková Silvia Kočiová Pavel Diviš Pavel Kopel Radek Veselý Veronika Pavliňáková Vojtěch Adam Lucy Vojtová Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds Nanomaterials tissue engineering drug release freeze-drying collagen chitosan selenium nanoparticles |
| author_facet |
Jana Dorazilová Johana Muchová Kristýna Šmerková Silvia Kočiová Pavel Diviš Pavel Kopel Radek Veselý Veronika Pavliňáková Vojtěch Adam Lucy Vojtová |
| author_sort |
Jana Dorazilová |
| title |
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds |
| title_short |
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds |
| title_full |
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds |
| title_fullStr |
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds |
| title_full_unstemmed |
Synergistic Effect of Chitosan and Selenium Nanoparticles on Biodegradation and Antibacterial Properties of Collagenous Scaffolds Designed for Infected Burn Wounds |
| title_sort |
synergistic effect of chitosan and selenium nanoparticles on biodegradation and antibacterial properties of collagenous scaffolds designed for infected burn wounds |
| publisher |
MDPI AG |
| series |
Nanomaterials |
| issn |
2079-4991 |
| publishDate |
2020-10-01 |
| description |
A highly porous scaffold is a desirable outcome in the field of tissue engineering. The porous structure mediates water-retaining properties that ensure good nutrient transportation as well as creates a suitable environment for cells. In this study, porous antibacterial collagenous scaffolds containing chitosan and selenium nanoparticles (SeNPs) as antibacterial agents were studied. The addition of antibacterial agents increased the application potential of the material for infected and chronic wounds. The morphology, swelling, biodegradation, and antibacterial activity of collagen-based scaffolds were characterized systematically to investigate the overall impact of the antibacterial additives. The additives visibly influenced the morphology, water‑retaining properties as well as the stability of the materials in the presence of collagenase enzymes. Even at concentrations as low as 5 ppm of SeNPs, modified polymeric scaffolds showed considerable inhibition activity towards Gram-positive bacterial strains such as <i>Staphylococcus aureus</i> and methicillin-resistant <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> in a dose-dependent manner. |
| topic |
tissue engineering drug release freeze-drying collagen chitosan selenium nanoparticles |
| url |
https://www.mdpi.com/2079-4991/10/10/1971 |
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