Genotyping of group A rotavirus samples from Brazilian children by probe hybridization

• Main Authors: D.D.P. Cardoso, M.L. Rácz, M.S.P. Azevedo, R.M.B. Martins, C.M.A. Soares
• Format: Article
• Language: English
• Published: Associação Brasileira de Divulgação Científica 2001-04-01
• Series: Brazilian Journal of Medical and Biological Research
• Subjects: rotavirus; G genotyping; VP7; oligonucleotides
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400005

The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil.