STRAIGHTFORWARD, ECONOMICAL PROCEDURES FOR MICROSCALE ELLMAN'S TEST FOR CHOLINESTERASE INHIBITION AND REACTIVATION

Straightforward, microscale, Ellman's cholinesterase test procedures are presented, useful for screening of novel compounds aiming at neurodegenerative diseases and antidotes towards nerve agents and pesticides. Herein, we detail the different procedure features and set forth guidelines on expe...

Full description

Bibliographic Details
Main Authors: Samir F. de A. Cavalcante, Daniel A. S. Kitagawa, Rafael B. Rodrigues, Monique Cardozo, Reuel L. de Paula, Ana Beatriz de A. Correa, Alessandro B. C. Simas
Format: Article
Language:English
Published: Sociedade Brasileira de Química
Series:Química Nova
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018001001192&lng=en&tlng=en
Description
Summary:Straightforward, microscale, Ellman's cholinesterase test procedures are presented, useful for screening of novel compounds aiming at neurodegenerative diseases and antidotes towards nerve agents and pesticides. Herein, we detail the different procedure features and set forth guidelines on experimental choices researchers may make. The detailed procedure lowers the costs for Ellman´s test, what is highly desirable for low-budget groups in science-lagging and developing countries.
ISSN:1678-7064