Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.

In insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural ins...

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Main Authors: Jianbai Liu, Han Wu, Jiequn Yi, Dingxin Jiang, Guren Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0217493
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spelling doaj-d8196883c7cb435d951328dcdef64c1a2021-03-03T20:37:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021749310.1371/journal.pone.0217493Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.Jianbai LiuHan WuJiequn YiDingxin JiangGuren ZhangIn insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural insect pests. However, the molecular mechanism of gustation in T. chilonis remains elusive. In this study, we found that T. chilonis adults had a preference for D-fructose and that D-fructose contributed to prolong longevity and improve fecundity. Then, We also isolated the full-length cDNA encoding candidate gustatory receptor (TchiGR43a) based on the transcriptome data of T. chilonis, and observed that the candidate gustatory receptor gene was expressed from the larval to adult stages. The expression levels of TchiGR43a were similar between female and male. A Xenopus oocyte expression system and two-electrode voltage-clamp recording further verified the function analysis of TchiGR43a. Electrophysiological results showed that TchiGR43a was exclusively tuned to D-fructose. By the studies of behaviour, molecular biology and electrophysiology in T. chilonis, our results lay a basic fundation of further study on the molecular mechanisms of gustatory reception and provide theoretical basis for the nutritional requirement of T. chilonis in biocontrol.https://doi.org/10.1371/journal.pone.0217493
collection DOAJ
language English
format Article
sources DOAJ
author Jianbai Liu
Han Wu
Jiequn Yi
Dingxin Jiang
Guren Zhang
spellingShingle Jianbai Liu
Han Wu
Jiequn Yi
Dingxin Jiang
Guren Zhang
Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
PLoS ONE
author_facet Jianbai Liu
Han Wu
Jiequn Yi
Dingxin Jiang
Guren Zhang
author_sort Jianbai Liu
title Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
title_short Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
title_full Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
title_fullStr Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
title_full_unstemmed Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis.
title_sort identification and functional characterization of d-fructose receptor in an egg parasitoid, trichogramma chilonis.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2019-01-01
description In insects, the gustatory system has a critical function not only in selecting food and feeding behaviours but also in growth and metabolism. Gustatory receptors play an irreplaceable role in insect gustatory signalling. Trichogramma chilonis is an effective biocontrol agent against agricultural insect pests. However, the molecular mechanism of gustation in T. chilonis remains elusive. In this study, we found that T. chilonis adults had a preference for D-fructose and that D-fructose contributed to prolong longevity and improve fecundity. Then, We also isolated the full-length cDNA encoding candidate gustatory receptor (TchiGR43a) based on the transcriptome data of T. chilonis, and observed that the candidate gustatory receptor gene was expressed from the larval to adult stages. The expression levels of TchiGR43a were similar between female and male. A Xenopus oocyte expression system and two-electrode voltage-clamp recording further verified the function analysis of TchiGR43a. Electrophysiological results showed that TchiGR43a was exclusively tuned to D-fructose. By the studies of behaviour, molecular biology and electrophysiology in T. chilonis, our results lay a basic fundation of further study on the molecular mechanisms of gustatory reception and provide theoretical basis for the nutritional requirement of T. chilonis in biocontrol.
url https://doi.org/10.1371/journal.pone.0217493
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