The Effect of Zearalenone on the Cytokine Environment, Oxidoreductive Balance and Metabolism in Porcine Ileal Peyer’s Patches

• Main Authors: Kazimierz Obremski, Wojciech Trybowski, Paweł Wojtacha, Magdalena Gajęcka, Józef Tyburski, Łukasz Zielonka
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: Toxins
• Subjects: zearalenone; pre-pubertal gilts; GALT; oxidative stress; cytokine; metabolism
• Online Access: https://www.mdpi.com/2072-6651/12/6/350

The aim of the present study was to determine the effect of zearalenone (ZEN), administered <i>per os</i> to gilts at doses equivalent to 50%, 100%, and 150% of no-observed-adverse-effect level (NOAEL) values for 14, 28, and 42 days during weaning, on changes in the parameters of the oxidoreductive balance, cytokine secretion, and basal metabolism in ileal Payer’s patches. Immunoenzymatic ELISA tests and biochemical methods were used to measure the concentrations of interleukin 1α, interleukin 1β, interleukin 12/23p40, interleukin 2, interferon γ, interleukin 4, interleukin 6, interleukin 8, tumor necrosis factor α, interleukin 10, transforming growth factor β, malondialdehyde, sulfhydryl groups, fructose, glucose, and proline, as well as the activity of peroxidase, superoxide dismutase and catalase. The study demonstrated that ZEN doses corresponding to 50%, 100%, and 150% of NOAEL values, i.e., 5 µg, 10 µg, and 15 µg ZEN/kg BW, respectively, have proinflammatory properties, exacerbate oxidative stress responses, and disrupt basal metabolism in ileal Payer’s patches in gilts.