Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles

Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles

Critical limb ischemia is one of the most advanced forms of peripheral artery disease, which seriously threat the human health and even cause amputation. In this study, we developed a fluorescent gelatin nanoparticle (FGNP) by covalent conjugation of the nanoparticles with two fluorogens, Cy7 and rh...

Full description

Bibliographic Details
Main Authors: Ju Zhang, Gang Wang, Duo Mao, Aitian Han, Nannan Xiao, Xin Qi, Dan Ding, Deling Kong
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Journal of Nanomaterials
Online Access:http://dx.doi.org/10.1155/2015/704817
id doaj-d767c8ce4735415ea16c6ffcbe28d1ef
record_format Article
spelling doaj-d767c8ce4735415ea16c6ffcbe28d1ef2020-11-24T22:43:08ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292015-01-01201510.1155/2015/704817704817Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin NanoparticlesJu Zhang0Gang Wang1Duo Mao2Aitian Han3Nannan Xiao4Xin Qi5Dan Ding6Deling Kong7State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaTianjin Union Medicine Center (Nankai University Affiliated Hospital), Clinical and Translation Medicine Lab, Tianjin 300121, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaState Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, ChinaCritical limb ischemia is one of the most advanced forms of peripheral artery disease, which seriously threat the human health and even cause amputation. In this study, we developed a fluorescent gelatin nanoparticle (FGNP) by covalent conjugation of the nanoparticles with two fluorogens, Cy7 and rhodamine B. The FGNPs have a volume average hydrodynamic diameter of about 168 nm, which also show low cytotoxicity against NIH/3T3 normal cells. The in vivo ischemia bioimaging studies in live mice and in ischemic limb slices demonstrate that the FGNPs can be preferentially accumulated to the ischemic site, which can thus serve as a safe and effective probe for targeted visualization of ischemia in the limb.http://dx.doi.org/10.1155/2015/704817
collection DOAJ
language English
format Article
sources DOAJ
author Ju Zhang
Gang Wang
Duo Mao
Aitian Han
Nannan Xiao
Xin Qi
Dan Ding
Deling Kong
spellingShingle Ju Zhang
Gang Wang
Duo Mao
Aitian Han
Nannan Xiao
Xin Qi
Dan Ding
Deling Kong
Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
Journal of Nanomaterials
author_facet Ju Zhang
Gang Wang
Duo Mao
Aitian Han
Nannan Xiao
Xin Qi
Dan Ding
Deling Kong
author_sort Ju Zhang
title Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
title_short Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
title_full Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
title_fullStr Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
title_full_unstemmed Targeted In Vivo Imaging of Mouse Hindlimb Ischemia Using Fluorescent Gelatin Nanoparticles
title_sort targeted in vivo imaging of mouse hindlimb ischemia using fluorescent gelatin nanoparticles
publisher Hindawi Limited
series Journal of Nanomaterials
issn 1687-4110
1687-4129
publishDate 2015-01-01
description Critical limb ischemia is one of the most advanced forms of peripheral artery disease, which seriously threat the human health and even cause amputation. In this study, we developed a fluorescent gelatin nanoparticle (FGNP) by covalent conjugation of the nanoparticles with two fluorogens, Cy7 and rhodamine B. The FGNPs have a volume average hydrodynamic diameter of about 168 nm, which also show low cytotoxicity against NIH/3T3 normal cells. The in vivo ischemia bioimaging studies in live mice and in ischemic limb slices demonstrate that the FGNPs can be preferentially accumulated to the ischemic site, which can thus serve as a safe and effective probe for targeted visualization of ischemia in the limb.
url http://dx.doi.org/10.1155/2015/704817
work_keys_str_mv AT juzhang targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT gangwang targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT duomao targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT aitianhan targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT nannanxiao targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT xinqi targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT danding targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
AT delingkong targetedinvivoimagingofmousehindlimbischemiausingfluorescentgelatinnanoparticles
_version_ 1725697444233084928

Similar Items