Experimental study of baicalein inhibiting high glucose-induced phenotypic transformation of vascular smooth muscle cells

• Main Authors: Wen Zhou, Qing-Song Wang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2021-04-01
• Series: Journal of Hainan Medical University
• Subjects: baicalein; high glucose; vascular smooth muscle cells; phenotypic transformationv
• Online Access: http://www.hnykdxxb.com/PDF/202108/01.pdf

Objective: To investigate the role of baicalein in phenotypic transformation of vascular smooth muscle cells induced by high glucose. Methods: Rat vascular smooth muscle cell experiments were divided into control group, baicalein group, high glucose group, high glucose plus baicalein group, real time quantitative PCR were used for mRNA analysis of -SMA, SM22- and OPN, Western blot were used for protein analysis of 毩-SMA, SM22-毩 and OPN. Results: Comparing the high glucose group and the high glucose plus baicalein group, the level of 毩-SMA mRNA in high glucose group was 0.419 ± 0.090, the level of 毩-SMA mRNA in high glucose plus baicalein group was 0.699 ± 0.079, the latter was 66.8% higher than the former. The level of 毩-SMA protein in high glucose group was 0.213 ± 0.034, the level of 毩-SMA protein in high glucose plus baicalein group was 0.393 ± 0.062, the latter was 84.5% higher than the former. Baicalein could significantly inhibit the down-regulation of 毩-SMA gene expression induced by high glucose (P <0.05). Comparing the high glucose group and the high glucose plus baicalein group, the level of SM22- mRNA in high glucose group was 0.369 ± 0.063, the level of SM22-毩 mRNA in high glucose plus baicalein group was 0.583 ± 0.049, the latter was 58.0% higher than the former. The level of SM22-毩 protein in high glucose group was 0.343 ± 0.047, the level of SM22- protein in high glucose plus baicalein group was 0.486 ± 0.051, the latter was 41.7% higher than the former. Baicalein could significantly inhibit the down-regulation of SM22-毩 gene expression induced by high glucose (P <0.05). Comparing the high glucose group and the high glucose plus baicalein group, the level of OPN mRNA in high glucose group was 2.023 ± 0.281, the level of OPN mRNA in high glucose plus baicalein group was 1.511 ± 0.091, the latter was 25.3% lower than the former. The level of OPN protein in high glucose group was 1.063 ± 0.132, the level of OPN protein in high glucose plus baicalein group was 0.761 ± 0.089, the latter was 28.4% lower than the former. Baicalein could significantly inhibit the up-regulation of OPN gene expression induced by high glucose (P <0.05). Conclusion: Baicalein can significantly inhibit the high glucose-induced phenotypic transformation of vascular smooth muscle cells from contractile phenotype to synthetic phenotype.