Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation
Asthma, emphysema, COVID-19 and other lung-impacting diseases cause the remodeling of tissue structural properties and can lead to changes in conducting pulmonary volume, viscoelasticity, and air flow distribution. Whole organ experimental inflation tests are commonly used to understand the impact o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-10-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.578762/full |
| id |
doaj-d74dd2392d95473093d4c99fd82d425d |
|---|---|
| record_format |
Article |
| spelling |
doaj-d74dd2392d95473093d4c99fd82d425d2020-11-25T03:39:15ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.578762578762Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure VentilationSamaneh Sattari0Crystal A. Mariano1Swathi Vittalbabu2Jalene V. Velazquez3Jalene V. Velazquez4Jessica Postma5Caleb Horst6Eric Teh7Tara M. Nordgren8Tara M. Nordgren9Mona Eskandari10Mona Eskandari11Mona Eskandari12Department of Mechanical Engineering, University of California, Riverside, Riverside, CA, United StatesDepartment of Mechanical Engineering, University of California, Riverside, Riverside, CA, United StatesDepartment of Mechanical Engineering, University of California, Riverside, Riverside, CA, United StatesBREATHE Center at the School of Medicine, University of California, Riverside, Riverside, CA, United StatesDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, United StatesCellScale Biomaterials Testing, Waterloo, ON, CanadaCellScale Biomaterials Testing, Waterloo, ON, CanadaCellScale Biomaterials Testing, Waterloo, ON, CanadaBREATHE Center at the School of Medicine, University of California, Riverside, Riverside, CA, United StatesDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, United StatesDepartment of Mechanical Engineering, University of California, Riverside, Riverside, CA, United StatesBREATHE Center at the School of Medicine, University of California, Riverside, Riverside, CA, United StatesDepartment of Bioengineering, University of California, Riverside, Riverside, CA, United StatesAsthma, emphysema, COVID-19 and other lung-impacting diseases cause the remodeling of tissue structural properties and can lead to changes in conducting pulmonary volume, viscoelasticity, and air flow distribution. Whole organ experimental inflation tests are commonly used to understand the impact of these modifications on lung mechanics. Here we introduce a novel, automated, custom-designed device for measuring the volume and pressure response of lungs, surpassing the capabilities of traditional machines and built to range size-scales to accommodate both murine and porcine tests. The software-controlled system is capable of constructing standardized continuous volume-pressure curves, while accounting for air compressibility, yielding consistent and reproducible measures while eliminating the need for pulmonary degassing. This device uses volume-control to enable viscoelastic whole lung macromechanical insights from rate dependencies and pressure-time curves. Moreover, the conceptual design of this device facilitates studies relating the phenomenon of diaphragm breathing and artificial ventilation induced by pushing air inside the lungs. System capabilities are demonstrated and validated via a comparative study between ex vivo murine lungs and elastic balloons, using various testing protocols. Volume-pressure curve comparisons with previous pressure-controlled systems yield good agreement, confirming accuracy. This work expands the capabilities of current lung experiments, improving scientific investigations of healthy and diseased pulmonary biomechanics. Ultimately, the methodologies demonstrated in the manufacturing of this system enable future studies centered on investigating viscoelasticity as a potential biomarker and improvements to patient ventilators based on direct assessment and comparisons of positive- and negative-pressure mechanics.https://www.frontiersin.org/articles/10.3389/fbioe.2020.578762/fulllung and respiratory mechanicspressure-volume curveviscoelasticitypositive and negative pressure ventilationCOVID-19pulmonary biomechanics |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Samaneh Sattari Crystal A. Mariano Swathi Vittalbabu Jalene V. Velazquez Jalene V. Velazquez Jessica Postma Caleb Horst Eric Teh Tara M. Nordgren Tara M. Nordgren Mona Eskandari Mona Eskandari Mona Eskandari |
| spellingShingle |
Samaneh Sattari Crystal A. Mariano Swathi Vittalbabu Jalene V. Velazquez Jalene V. Velazquez Jessica Postma Caleb Horst Eric Teh Tara M. Nordgren Tara M. Nordgren Mona Eskandari Mona Eskandari Mona Eskandari Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation Frontiers in Bioengineering and Biotechnology lung and respiratory mechanics pressure-volume curve viscoelasticity positive and negative pressure ventilation COVID-19 pulmonary biomechanics |
| author_facet |
Samaneh Sattari Crystal A. Mariano Swathi Vittalbabu Jalene V. Velazquez Jalene V. Velazquez Jessica Postma Caleb Horst Eric Teh Tara M. Nordgren Tara M. Nordgren Mona Eskandari Mona Eskandari Mona Eskandari |
| author_sort |
Samaneh Sattari |
| title |
Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation |
| title_short |
Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation |
| title_full |
Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation |
| title_fullStr |
Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation |
| title_full_unstemmed |
Introducing a Custom-Designed Volume-Pressure Machine for Novel Measurements of Whole Lung Organ Viscoelasticity and Direct Comparisons Between Positive- and Negative-Pressure Ventilation |
| title_sort |
introducing a custom-designed volume-pressure machine for novel measurements of whole lung organ viscoelasticity and direct comparisons between positive- and negative-pressure ventilation |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Bioengineering and Biotechnology |
| issn |
2296-4185 |
| publishDate |
2020-10-01 |
| description |
Asthma, emphysema, COVID-19 and other lung-impacting diseases cause the remodeling of tissue structural properties and can lead to changes in conducting pulmonary volume, viscoelasticity, and air flow distribution. Whole organ experimental inflation tests are commonly used to understand the impact of these modifications on lung mechanics. Here we introduce a novel, automated, custom-designed device for measuring the volume and pressure response of lungs, surpassing the capabilities of traditional machines and built to range size-scales to accommodate both murine and porcine tests. The software-controlled system is capable of constructing standardized continuous volume-pressure curves, while accounting for air compressibility, yielding consistent and reproducible measures while eliminating the need for pulmonary degassing. This device uses volume-control to enable viscoelastic whole lung macromechanical insights from rate dependencies and pressure-time curves. Moreover, the conceptual design of this device facilitates studies relating the phenomenon of diaphragm breathing and artificial ventilation induced by pushing air inside the lungs. System capabilities are demonstrated and validated via a comparative study between ex vivo murine lungs and elastic balloons, using various testing protocols. Volume-pressure curve comparisons with previous pressure-controlled systems yield good agreement, confirming accuracy. This work expands the capabilities of current lung experiments, improving scientific investigations of healthy and diseased pulmonary biomechanics. Ultimately, the methodologies demonstrated in the manufacturing of this system enable future studies centered on investigating viscoelasticity as a potential biomarker and improvements to patient ventilators based on direct assessment and comparisons of positive- and negative-pressure mechanics. |
| topic |
lung and respiratory mechanics pressure-volume curve viscoelasticity positive and negative pressure ventilation COVID-19 pulmonary biomechanics |
| url |
https://www.frontiersin.org/articles/10.3389/fbioe.2020.578762/full |
| work_keys_str_mv |
AT samanehsattari introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT crystalamariano introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT swathivittalbabu introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT jalenevvelazquez introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT jalenevvelazquez introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT jessicapostma introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT calebhorst introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT ericteh introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT taramnordgren introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT taramnordgren introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT monaeskandari introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT monaeskandari introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation AT monaeskandari introducingacustomdesignedvolumepressuremachinefornovelmeasurementsofwholelungorganviscoelasticityanddirectcomparisonsbetweenpositiveandnegativepressureventilation |
| _version_ |
1724540120339578880 |