Gamma-Aminobutyric Acid Production Using Immobilized Glutamate Decarboxylase Followed by Downstream Processing with Cation Exchange Chromatography

Gamma-Aminobutyric Acid Production Using Immobilized Glutamate Decarboxylase Followed by Downstream Processing with Cation Exchange Chromatography

We have developed a gamma-aminobutyric acid (GABA) production technique using his-tag mediated immobilization of Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamate to GABA. The GAD was obtained at 1.43 g/L from GAD-overexpressed E. coli ferme...

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Bibliographic Details
Main Authors: Hongweon Lee, Eun Gyo Lee, Joon-Ki Jung, Yeon-Gu Kim, Jungoh Ahn, Seungwoon Lee
Format: Article
Language:English
Published: MDPI AG 2013-01-01
Series:International Journal of Molecular Sciences
Subjects:
gamma aminobutyric acid
glutamate decarboxylase
glutamic acid
immobilization
cation exchange chromatography
Online Access:http://www.mdpi.com/1422-0067/14/1/1728

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http://www.mdpi.com/1422-0067/14/1/1728

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