Evaluation of Sequencing Library Preparation Protocols for Viral Metagenomic Analysis from Pristine Aquifer Groundwaters

Evaluation of Sequencing Library Preparation Protocols for Viral Metagenomic Analysis from Pristine Aquifer Groundwaters

Viral ecology of terrestrial habitats is yet-to be extensively explored, in particular the terrestrial subsurface. One problem in obtaining viral sequences from groundwater aquifer samples is the relatively low amount of virus particles. As a result, the amount of extracted DNA may not be sufficient...

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Bibliographic Details
Main Authors: René Kallies, Martin Hölzer, Rodolfo Brizola Toscan, Ulisses Nunes da Rocha, John Anders, Manja Marz, Antonis Chatzinotas
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Viruses
Subjects:
viral metagenome
groundwater
aquifer
AquaDiva
sequencing library preparation
Online Access:https://www.mdpi.com/1999-4915/11/6/484

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