The Intracellular Distribution of Folate Derivatives in Pea Leaves

The Intracellular Distribution of Folate Derivatives in Pea Leaves

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After hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.1...

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Main Authors: Chan Sherwin Y., Cossins Edwin A.
Format: Article
Language:English
Published: De Gruyter 2003-08-01
Series:Pteridines
Subjects:
folates
mitochondria
chloroplasts
greening
photorespiration
high performance liquid chromatography
pisum sativum l
Online Access:https://doi.org/10.1515/pteridines.2003.14.3.17
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spelling doaj-d738a399560446da98b374337dd206492021-09-05T14:00:01ZengDe GruyterPteridines0933-48072195-47202003-08-01143172610.1515/pteridines.2003.14.3.17The Intracellular Distribution of Folate Derivatives in Pea LeavesChan Sherwin Y.0Cossins Edwin A.1Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, CanadaAfter hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.17+0.01 nmol folate mg'1 protein that was predominantly methylated and associated with the cytosolic fraction. Percoli gradient-purified mitochondria and chloroplasls contained 11,0±2.3% and 8.4±0.6% of cellular folate, respectively. Mitochondrial folates (0.47+0.11 nmol folate mg"' protein) were predominately (90%) formylatcd and unsubstituted. In contrast, chloroplasts contained a less concentrated (0.02±0.001 nmol folate mg"' protein) folate pool consisting of approximately 30% methylated folate.https://doi.org/10.1515/pteridines.2003.14.3.17folatesmitochondriachloroplastsgreeningphotorespirationhigh performance liquid chromatographypisum sativum l
collection DOAJ
language English
format Article
sources DOAJ
author Chan Sherwin Y.
Cossins Edwin A.
spellingShingle Chan Sherwin Y.
Cossins Edwin A.
The Intracellular Distribution of Folate Derivatives in Pea Leaves
Pteridines
folates
mitochondria
chloroplasts
greening
photorespiration
high performance liquid chromatography
pisum sativum l
author_facet Chan Sherwin Y.
Cossins Edwin A.
author_sort Chan Sherwin Y.
title The Intracellular Distribution of Folate Derivatives in Pea Leaves
title_short The Intracellular Distribution of Folate Derivatives in Pea Leaves
title_full The Intracellular Distribution of Folate Derivatives in Pea Leaves
title_fullStr The Intracellular Distribution of Folate Derivatives in Pea Leaves
title_full_unstemmed The Intracellular Distribution of Folate Derivatives in Pea Leaves
title_sort intracellular distribution of folate derivatives in pea leaves
publisher De Gruyter
series Pteridines
issn 0933-4807
2195-4720
publishDate 2003-08-01
description After hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.17+0.01 nmol folate mg'1 protein that was predominantly methylated and associated with the cytosolic fraction. Percoli gradient-purified mitochondria and chloroplasls contained 11,0±2.3% and 8.4±0.6% of cellular folate, respectively. Mitochondrial folates (0.47+0.11 nmol folate mg"' protein) were predominately (90%) formylatcd and unsubstituted. In contrast, chloroplasts contained a less concentrated (0.02±0.001 nmol folate mg"' protein) folate pool consisting of approximately 30% methylated folate.
topic folates
mitochondria
chloroplasts
greening
photorespiration
high performance liquid chromatography
pisum sativum l
url https://doi.org/10.1515/pteridines.2003.14.3.17
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