The Intracellular Distribution of Folate Derivatives in Pea Leaves
After hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.1...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2003-08-01
|
| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.2003.14.3.17 |
| Summary: | After hydrolysis of polyglutamate derivatives, leaf extracts of pea (Pisum sativum L.) seedlings were examined for individual folates using high performance liquid chromatography and microbiological assays employing Lactobacillus rhamnosus and Pediococcus acidilactici. 14-day seedlings contained 0.17+0.01 nmol folate mg'1 protein that was predominantly methylated and associated with the cytosolic fraction. Percoli gradient-purified mitochondria and chloroplasls contained 11,0±2.3% and 8.4±0.6% of cellular folate, respectively. Mitochondrial folates (0.47+0.11 nmol folate mg"' protein) were predominately (90%) formylatcd and unsubstituted. In contrast, chloroplasts contained a less concentrated (0.02±0.001 nmol folate mg"' protein) folate pool consisting of approximately 30% methylated folate. |
|---|---|
| ISSN: | 0933-4807 2195-4720 |