beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones
<p>Abstract</p> <p>Several myelin-associated factors that inhibit axon growth of mature neurons, including Nogo66, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp), can associate with a common GPI-linked protein Nogo-66 receptor (NgR). Accumulatin...
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Series: | Molecular Brain |
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doaj-d72a81ad72d24ea2b1762493e1a699372020-11-24T21:54:54ZengBMCMolecular Brain1756-66062008-10-01111010.1186/1756-6606-1-10beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth conesGoh Eyleen LKYoung JuKuwako KenichiroTessier-Lavigne MarcHe ZhigangGriffin John WMing Guo-li<p>Abstract</p> <p>Several myelin-associated factors that inhibit axon growth of mature neurons, including Nogo66, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp), can associate with a common GPI-linked protein Nogo-66 receptor (NgR). Accumulating evidence suggests that myelin inhibitors also signal through unknown NgR-independent mechanisms. Here we show that MAG, a RGD tri-peptide containing protein, forms a complex with β1-integrin to mediate axonal growth cone turning responses of several neuronal types. Mutations that alter the RGD motif in MAG or inhibition of β1-integrin function, but not removal of NgRs, abolish these MAG-dependent events. In contrast, OMgp-induced repulsion is not affected by inhibition of b1-integrin function. We further show that MAG stimulates tyrosine phosphorylation of focal adhesion kinase (FAK), which in turn is required for MAG-induced growth cone turning. These studies identify β1-integrin as a specific mediator for MAG in growth cone turning responses, acting through FAK activation.</p> http://www.molecularbrain.com/content/1/1/10 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Goh Eyleen LK Young Ju Kuwako Kenichiro Tessier-Lavigne Marc He Zhigang Griffin John W Ming Guo-li |
spellingShingle |
Goh Eyleen LK Young Ju Kuwako Kenichiro Tessier-Lavigne Marc He Zhigang Griffin John W Ming Guo-li beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones Molecular Brain |
author_facet |
Goh Eyleen LK Young Ju Kuwako Kenichiro Tessier-Lavigne Marc He Zhigang Griffin John W Ming Guo-li |
author_sort |
Goh Eyleen LK |
title |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
title_short |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
title_full |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
title_fullStr |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
title_full_unstemmed |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
title_sort |
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones |
publisher |
BMC |
series |
Molecular Brain |
issn |
1756-6606 |
publishDate |
2008-10-01 |
description |
<p>Abstract</p> <p>Several myelin-associated factors that inhibit axon growth of mature neurons, including Nogo66, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp), can associate with a common GPI-linked protein Nogo-66 receptor (NgR). Accumulating evidence suggests that myelin inhibitors also signal through unknown NgR-independent mechanisms. Here we show that MAG, a RGD tri-peptide containing protein, forms a complex with β1-integrin to mediate axonal growth cone turning responses of several neuronal types. Mutations that alter the RGD motif in MAG or inhibition of β1-integrin function, but not removal of NgRs, abolish these MAG-dependent events. In contrast, OMgp-induced repulsion is not affected by inhibition of b1-integrin function. We further show that MAG stimulates tyrosine phosphorylation of focal adhesion kinase (FAK), which in turn is required for MAG-induced growth cone turning. These studies identify β1-integrin as a specific mediator for MAG in growth cone turning responses, acting through FAK activation.</p> |
url |
http://www.molecularbrain.com/content/1/1/10 |
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