Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomater...

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Main Authors:	Jasmina Vidic, Priya Vizzini, Marisa Manzano, Devon Kavanaugh, Nalini Ramarao, Milica Zivkovic, Vasa Radonic, Nikola Knezevic, Ioanna Giouroudi, Ivana Gadjanski
Format:	Article
Language:	English
Published:	MDPI AG 2019-03-01
Series:	Sensors
Subjects:	biosensor point-of-need DNA detection DNA/RNA extraction DNA amplification microfluidic food security
Online Access:	http://www.mdpi.com/1424-8220/19/5/1100

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spelling	doaj-d727189c31cb4692bcc42bd9827a21d12020-11-24T21:51:13ZengMDPI AGSensors1424-82202019-03-01195110010.3390/s19051100s19051100Point-of-Need DNA Testing for Detection of Foodborne Pathogenic BacteriaJasmina Vidic0Priya Vizzini1Marisa Manzano2Devon Kavanaugh3Nalini Ramarao4Milica Zivkovic5Vasa Radonic6Nikola Knezevic7Ioanna Giouroudi8Ivana Gadjanski9Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceDipartimento di Scienze AgroAlimentari, Ambientali e Animali, Università di Udine, 33100 Udine, ItalyMicalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceMicalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceInstitute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, 11000 Belgrade, SerbiaBioSense—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, SerbiaBioSense—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, SerbiaBioSense—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, SerbiaBioSense—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, SerbiaFoodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.http://www.mdpi.com/1424-8220/19/5/1100biosensorpoint-of-need DNA detectionDNA/RNA extractionDNA amplificationmicrofluidicfood security
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jasmina Vidic Priya Vizzini Marisa Manzano Devon Kavanaugh Nalini Ramarao Milica Zivkovic Vasa Radonic Nikola Knezevic Ioanna Giouroudi Ivana Gadjanski
spellingShingle	Jasmina Vidic Priya Vizzini Marisa Manzano Devon Kavanaugh Nalini Ramarao Milica Zivkovic Vasa Radonic Nikola Knezevic Ioanna Giouroudi Ivana Gadjanski Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria Sensors biosensor point-of-need DNA detection DNA/RNA extraction DNA amplification microfluidic food security
author_facet	Jasmina Vidic Priya Vizzini Marisa Manzano Devon Kavanaugh Nalini Ramarao Milica Zivkovic Vasa Radonic Nikola Knezevic Ioanna Giouroudi Ivana Gadjanski
author_sort	Jasmina Vidic
title	Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
title_short	Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
title_full	Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
title_fullStr	Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
title_full_unstemmed	Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria
title_sort	point-of-need dna testing for detection of foodborne pathogenic bacteria
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2019-03-01
description	Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.
topic	biosensor point-of-need DNA detection DNA/RNA extraction DNA amplification microfluidic food security
url	http://www.mdpi.com/1424-8220/19/5/1100
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Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

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