Target-selective joint polymerase chain reaction: A robust and rapid method for high-throughput production of recombinant monoclonal antibodies from single cells

<p>Abstract</p> <p>Background</p> <p>During the development of a therapeutic antibody, large numbers of monoclonal antibodies are required to screen for those that are best suited for the desired activity. Although the single cell-based immunoglobulin variable gene clon...

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Bibliographic Details
Main Authors: Isobe Masaharu, Kurosawa Nobuyuki, Yoshioka Megumi
Format: Article
Language:English
Published: BMC 2011-07-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/11/75