| Summary: | A big challenge for today’s industry is antimicrobial preservation and the safety of food. An effective solution to this problem can be a modern invention such as antimicrobial packaging. In the presented research the antimicrobial activity of two new active films incorporating silver, as IONPURE IPL, and ethyl lauroyl arginate (LAE) were evaluated, by employing a low-density polyethylene (LDPE) matrix and a biofilm material, respectively. Additionally, LAE was also incorporated into polystyrene (PS) pads by two different methods: by spraying and by immersion of the PS pads into an aqueous LAE solution. LDPE films containing silver did not show any antimicrobial activity against <i>Escherichia coli</i> and <i>Aspergillus flavus,</i> whereas the biofilm containing LAE reduced the growth of <i>Salmonella enterica</i> but did not inhibit <i>Aspergillus flavus</i>. The active PS pads, both sprayed and immersed in LAE solution, also showed antimicrobial activity, causing a reduction of 99.99% of <i>Pseudomonas putida</i> growth. Thermal treatment at 180 °C for 6 and 15 min did not modify the antimicrobial activity of LAE against <i>Salmonella enterica.</i> Moreover, inductively coupled plasma-mass spectrometry (ICP-MS) analysis was performed to check the migration of silver from developed material intended for food packaging applications into food simulant.
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