Live-Cell Imaging and Functional Dissection of Xist RNA Reveal Mechanisms of X Chromosome Inactivation and Reactivation

Live-Cell Imaging and Functional Dissection of Xist RNA Reveal Mechanisms of X Chromosome Inactivation and Reactivation

Summary: We double-tagged Xist (inactivated X chromosome-specific transcript), a prototype long non-coding RNA pivotal for X chromosome inactivation (XCI), using the programmable RNA sequence binding domain of Pumilio protein, one tag for live-cell imaging and the other replacing A-repeat (a critica...

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Main Authors: Norbert Ha, Lan-Tian Lai, Rosi Chelliah, Yashu Zhen, Seet Pei Yi Vanessa, Soak-Kuan Lai, Hoi-Yeung Li, Alexander Ludwig, Sara Sandin, Lingyi Chen, Li-Feng Zhang
Format: Article
Language:English
Published: Elsevier 2018-10-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004218301421

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http://www.sciencedirect.com/science/article/pii/S2589004218301421

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