A Comparative Evaluation on Antimicrobial Effect of Honey, Neem Leaf Extract and Sodium Hypochlorite as Intracanal Irrigant: An Ex-Vivo Study
Introduction: The major determinant of the success of root canal treatment depends on meticulous disinfection of the root canal using intracanal irrigants. The most commonly used root canal irrigant is sodium hypochlorite which has disadvantages of cytotoxicity and unpleasant taste. So there is...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2016-08-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/8311/19268_CE(EK)_F(AK)_PF1(NEAK)_PFA(AK)_PF2(PAG).pdf |
Summary: | Introduction: The major determinant of the success of root
canal treatment depends on meticulous disinfection of the root
canal using intracanal irrigants. The most commonly used root
canal irrigant is sodium hypochlorite which has disadvantages
of cytotoxicity and unpleasant taste. So there is a need to
identify a more biocompatible root canal irrigant.
Aim: The aim of this ex-vivo study was to evaluate the efficacy
of 40% honey, 100% neem leaf extract and 5.25% sodium
hypochlorite as an intracanal irrigant against the isolated
microorganisms from infected root canal.
Materials and Methods: The samples were collected from
infected root canals of 60 primary molar teeth indicated for
pulpectomy. Alpha hemolytic Streptococci, gram negative bacilli,
Candida, Staphylococci, Lactobacilli, Enterococci, Spore bearing
gram positive bacilli and Micrococci were the microorganisms
isolated from the samples. The zone of inhibition against the
microbial growth was measured by agar well diffusion method.
Statistical analysis was done by Repeated Analysis of Variance
(ANOVA) and Bonferroni method.
Results: Statistical analysis showed that the means of the zones
of inhibition measured in this study were 18.56mm, 2.09mm and
1.62mm for sodium hypochlorite, 100% neem leaf extract and
40% honey respectively. The significance was greater between
sodium hypochlorite and the other two agents as p-value was
<0.001.
Conclusion: The results indicated that 5.25% sodium
hypochlorite is more effective as root canal irrigant when
compared with 100% neem leaf extract and 40% honey. It
was also observed that 100% neem leaf extract has greater
antimicrobial effect than 40% honey. |
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ISSN: | 2249-782X 0973-709X |