Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Abstract Background Thermostable lipases from microbial sources have been substantially overexpressed in E. coli, however, these enzymes are often produced with low-level enzymatic activity and mainly in the form of inclusion bodies. Several studies have reported that the secretory production of rec...

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Bibliographic Details
Main Authors: Weiqing Zhang, Jing Lu, Shuwen Zhang, Lu Liu, Xiaoyang Pang, Jiaping Lv
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Microbial Cell Factories
Subjects:
Lipase
Signal peptide
Fusion expression
E. coli
DsbA
Online Access:http://link.springer.com/article/10.1186/s12934-018-0894-y

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http://link.springer.com/article/10.1186/s12934-018-0894-y

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