ABOUT RENAL CAPSULE TUMOUR HISTOGENESIS, INDUCED BY 1,2-DIMETHYLHYDRAZINE IN CBA MICE

• Main Authors: O. V. Morozova, Е. Е. Antoshina, V. N. Manskikh
• Format: Article
• Language: Russian
• Published: ABV-press 2017-12-01
• Series: Uspehi Molekulârnoj Onkologii
• Subjects: 1.2-dimethylhydrazine; mouse сва; renal capsule; hemangiosarcoma; histogenesis; immunohistochemistry
• Online Access: https://umo.abvpress.ru/jour/article/view/117

Background. It is accepted that 1,2-dimethylhydrazine  (DMH)  induced androgen-dependent hemangiosarcoma (HAS)  of fibrous renal capsule (RC) in CBA male mice. This experimental model is used to study hormone-dependent tumors. It is considered that RС (site of HAS growth) is avaskular. For clarifying the nature of such paradoxical phenomen we studied the tumour histogenesis.Materials and methods. CBA male mice received 15 subcutaneous injections of DMH. The duration of the experiment was 50 weeks. From RC whole-mounted preparations and paraffin sections were made. Immunohistochemistry method with antibody against endothelium and stem cells CD34 were used to investigate the endothelium cell precursors.Results. We have found single CD34 well expressive cells and capillary structures in intact RC. Nevertheless on the initial stages of HAS growth the CD34 expression was markedly weaker or completely absent compared with intact RC. In the late HAS develop the intensive CD34 expression was detected.Conclusions. In summary, for the first time we have shown that murine fibrous RC is not completely avascular education, as it was supposed earlier. In the HAS morphogenesis process the phase alterations of CD34 antigen expression were found.