Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch

Identifying conformational changes with site-directed spin labeling reveals that the GTPase domain of HydF is a molecular switch

Abstract [FeFe]-hydrogenases catalyse the reduction of protons to hydrogen at a complex 2Fe[4Fe4S] center called H-cluster. The assembly of this active site is a multistep process involving three proteins, HydE, HydF and HydG. According to the current models, HydF has the key double role of scaffold...

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Bibliographic Details
Main Authors: Laura Galazzo, Lorenzo Maso, Edith De Rosa, Marco Bortolus, Davide Doni, Laura Acquasaliente, Vincenzo De Filippis, Paola Costantini, Donatella Carbonera
Format: Article
Language:English
Published: Nature Publishing Group 2017-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-01886-y

Internet

https://doi.org/10.1038/s41598-017-01886-y

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