Genome Walking by Next Generation Sequencing Approaches

Genome Walking by Next Generation Sequencing Approaches

Genome Walking (GW) comprises a number of PCR-based methods for the identification of nucleotide sequences flanking known regions. The different methods have been used for several purposes: from de novo sequencing, useful for the identification of unknown regions, to the characterization of insertio...

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Bibliographic Details
Main Authors: Luigi R. Ceci, Immacolata Fanizza, Antonio Placido, Alessandra Costanza, Claudia Leoni, Mariateresa Volpicella
Format: Article
Language:English
Published: MDPI AG 2012-10-01
Series:Biology
Subjects:
genome walking
insertional mutagenesis
next generation sequencing
functional genomics
gene therapy
transposon tagging
454-pyrosequencing
Illumina
SOLiD
Ion Torrent
Online Access:http://www.mdpi.com/2079-7737/1/3/495

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