An inexpensive microscopy system for microfluidic studies in budding yeast

Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent deve...

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Main Authors:	Kenneth L. Chen, Toby N. Ven, Matthew M. Crane, Dexter E. Chen, Yen-Chi Feng, Nozomi Suzuki, Adam E. Russell, Diogo de Moraes, Matt Kaeberlein
Format:	Article
Language:	English
Published:	KeAi Communications Co., Ltd. 2019-01-01
Series:	Translational Medicine of Aging
Online Access:	http://www.sciencedirect.com/science/article/pii/S2468501119300069

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spelling	doaj-d581a5beefe545e9aa0b3ffef23869422021-04-02T11:54:34ZengKeAi Communications Co., Ltd.Translational Medicine of Aging2468-50112019-01-0135256An inexpensive microscopy system for microfluidic studies in budding yeastKenneth L. Chen0Toby N. Ven1Matthew M. Crane2Dexter E. Chen3Yen-Chi Feng4Nozomi Suzuki5Adam E. Russell6Diogo de Moraes7Matt Kaeberlein8Department of Pathology, School of Medicine, University of Washington, Seattle, WA, USA; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, WA, USA; Medical Scientist Training Program, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USADepartment of Pathology, School of Medicine, University of Washington, Seattle, WA, USA; Department of Genome Sciences, School of Medicine, University of Washington, Seattle, WA, USA; Corresponding author. Department of Pathology, School of Medicine, University of Washington, Seattle, WA, USA.Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast. Keywords: Yeast, Microfluidics, Microscopy, Replicative aging, Microdissection, Lifespan, 3D printing, Arduinohttp://www.sciencedirect.com/science/article/pii/S2468501119300069
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kenneth L. Chen Toby N. Ven Matthew M. Crane Dexter E. Chen Yen-Chi Feng Nozomi Suzuki Adam E. Russell Diogo de Moraes Matt Kaeberlein
spellingShingle	Kenneth L. Chen Toby N. Ven Matthew M. Crane Dexter E. Chen Yen-Chi Feng Nozomi Suzuki Adam E. Russell Diogo de Moraes Matt Kaeberlein An inexpensive microscopy system for microfluidic studies in budding yeast Translational Medicine of Aging
author_facet	Kenneth L. Chen Toby N. Ven Matthew M. Crane Dexter E. Chen Yen-Chi Feng Nozomi Suzuki Adam E. Russell Diogo de Moraes Matt Kaeberlein
author_sort	Kenneth L. Chen
title	An inexpensive microscopy system for microfluidic studies in budding yeast
title_short	An inexpensive microscopy system for microfluidic studies in budding yeast
title_full	An inexpensive microscopy system for microfluidic studies in budding yeast
title_fullStr	An inexpensive microscopy system for microfluidic studies in budding yeast
title_full_unstemmed	An inexpensive microscopy system for microfluidic studies in budding yeast
title_sort	inexpensive microscopy system for microfluidic studies in budding yeast
publisher	KeAi Communications Co., Ltd.
series	Translational Medicine of Aging
issn	2468-5011
publishDate	2019-01-01
description	Recently, microfluidic technologies have been developed to allow higher throughput collection of yeast replicative lifespan data. Adoption of these devices has been limited, in part, due to the high cost of the motorized microscopy instrumentation from mainline manufacturers. Inspired by recent development of open source microscopy hardware and software, we developed minimal-cost hardware attachments to provide long-term focus stabilization for lower-cost microscopes and open source software to manage concurrent time-lapse image acquisition from multiple microscopes. We hope that these tools will help spur the wider adoption of microfluidic technologies for the study of aging in yeast. Keywords: Yeast, Microfluidics, Microscopy, Replicative aging, Microdissection, Lifespan, 3D printing, Arduino
url	http://www.sciencedirect.com/science/article/pii/S2468501119300069
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An inexpensive microscopy system for microfluidic studies in budding yeast

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